Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/95349
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Type: | Journal article |
Title: | Genetic analysis of tolerance to the root lesion nematode Pratylenchus neglectus in the legume Medicago littoralis |
Author: | Oldach, K. Peck, D. Nair, R. Sokolova, M. Harris, J. Bogacki, P. Ballard, R. |
Citation: | BMC Plant Biology, 2014; 14(1):100-1-100-11 |
Publisher: | Biomed Central |
Issue Date: | 2014 |
ISSN: | 1471-2229 1471-2229 |
Statement of Responsibility: | Klaus H Oldach, David M Peck, Ramakrishnan M Nair, Maria Sokolova, John Harris, Paul Bogacki, and Ross Ballard |
Abstract: | BACKGROUND: The nematode Pratylenchus neglectus has a wide host range and is able to feed on the root systems of cereals, oilseeds, grain and pasture legumes. Under the Mediterranean low rainfall environments of Australia, annual Medicago pasture legumes are used in rotation with cereals to fix atmospheric nitrogen and improve soil parameters. Considerable efforts are being made in breeding programs to improve resistance and tolerance to Pratylenchus neglectus in the major crops wheat and barley, which makes it vital to develop appropriate selection tools in medics. RESULTS: A strong source of tolerance to root damage by the root lesion nematode (RLN) Pratylenchus neglectus had previously been identified in line RH-1 (strand medic, M. littoralis). Using RH-1, we have developed a single seed descent (SSD) population of 138 lines by crossing it to the intolerant cultivar Herald. After inoculation, RLN-associated root damage clearly segregated in the population. Genetic analysis was performed by constructing a genetic map using simple sequence repeat (SSR) and gene-based SNP markers. A highly significant quantitative trait locus (QTL), QPnTolMl.1, was identified explaining 49% of the phenotypic variation in the SSD population. All SSRs and gene-based markers in the QTL region were derived from chromosome 1 of the sequenced genome of the closely related species M. truncatula. Gene-based markers were validated in advanced breeding lines derived from the RH-1 parent and also a second RLN tolerance source, RH-2 (M. truncatula ssp. tricycla). Comparative analysis to sequenced legume genomes showed that the physical QTL interval exists as a synteny block in Lotus japonicus, common bean, soybean and chickpea. Furthermore, using the sequenced genome information of M. truncatula, the QTL interval contains 55 genes out of which five are discussed as potential candidate genes responsible for the mapped tolerance. CONCLUSION: The closely linked set of SNP-based PCR markers is directly applicable to select for two different sources of RLN tolerance in breeding programs. Moreover, genome sequence information has allowed proposing candidate genes for further functional analysis and nominates QPnTolMl.1 as a target locus for RLN tolerance in economically important grain legumes, e.g. chickpea. |
Keywords: | Pasture; RLN; Root disease; Gene-based markers; Genetic map; Comparative analysis; Candidate genes |
Rights: | © 2014 Oldach et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
DOI: | 10.1186/1471-2229-14-100 |
Published version: | http://dx.doi.org/10.1186/1471-2229-14-100 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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hdl_95349.pdf | Published version | 1.23 MB | Adobe PDF | View/Open |
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