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https://hdl.handle.net/2440/9291
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Type: | Journal article |
Title: | Chimeric caspase molecules with potent cell killing activity in apoptosis-resistant cells |
Author: | Shearwin-Whyatt, L. Baliga, B. Doumanis, J. Kumar, S. |
Citation: | Biochemical and Biophysical Research Communications, 2001; 282(5):1114-1119 |
Publisher: | Academic Press Inc |
Issue Date: | 2001 |
ISSN: | 0006-291X 1090-2104 |
Statement of Responsibility: | Linda Shearwin-Whyatt, Belinda Baliga, Joanna Doumanis and Sharad Kumar |
Abstract: | Cellular defects which prevent apoptotic cell death can result in the generation of hyperproliferative disorders and can prevent the effective treatment of such diseases. The majority of cellular defects which result in apoptosis resistance lie upstream of caspase activation. We have described chimeric caspase molecules consisting of the prodomain of caspase-2 fused to the amino terminus of caspase-3, and which are tagged at the carboxyl terminus with green fluorescent protein (GFP) to allow direct visualisation of transfected cells. Here we show that these chimeric caspase molecules possess potent, rapid cell-killing activity in cell lines which display a range of defects resulting in apoptosis resistance. |
Keywords: | COS Cells Tumor Cells, Cultured Animals Humans Leukemia, T-Cell Osteosarcoma Caspases Luminescent Proteins Green Fluorescent Proteins Recombinant Fusion Proteins Transfection Drug Resistance, Multiple Apoptosis Cell Survival Drug Resistance, Neoplasm Caspase 3 Caspase 2 Leukemia, Myelogenous, Chronic, BCR-ABL Positive ATP Binding Cassette Transporter, Subfamily B, Member 1 |
Description: | Copyright © 2001 Academic Press. All rights reserved. |
DOI: | 10.1006/bbrc.2001.4699 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description#description |
Published version: | http://dx.doi.org/10.1006/bbrc.2001.4699 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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