Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/81765
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Type: | Journal article |
Title: | Effects of differing oocyte-secreted factors during mouse in vitro maturation on subsequent embryo and fetal development |
Author: | Sudiman, J. Ritter, L. Inglis, D. Wang, X. Chan, K. Mottershead, D. Robertson, D. Thompson, J. Gilchrist, R. |
Citation: | Journal of Assisted Reproduction and Genetics, 2014; 31(3):295-306 |
Publisher: | Kluwer Academic/Plenum Publ |
Issue Date: | 2014 |
ISSN: | 1058-0468 1573-7330 |
Organisation: | Robinson Institute |
Statement of Responsibility: | J. Sudiman & L. J. Ritter & D. K. Feil & X.Wang & K. Chan & D. G. Mottershead & D. M. Robertson & J. G. Thompson & R. B. Gilchrist |
Abstract: | Purpose We hypothesised that varying native oocyte-secreted factor (OSF) exposure or using different recombinant OSF peptides would have differential effects on post-in vitro maturation (IVM) embryo and fetal development. Methods Mouse cumulus oocyte complexes (COCs) were treated with the purified mature domain of GDF9 and/or BMP15 or were co-cultured with denuded oocytes (DOs) from 0 h or 3 h of IVM. DOs were matured for 3 h as either intact COCs+/-FSH before denuding, or as DOs + FSH. COCs were fertilised and blastocyst development was assessed on days 5 and 6, and either differentially stained for ICM numbers or vitrified/warmed embryos were transferred to recipients to assess implantation and fetal rates. Results No improvement in embryo development was observed with the addition of GDF9 and/or BMP15 to IVM. In contrast, embryos derived from COCs co-cultured with DOs had significantly improved blastocyst rates and ICM numbers compared to controls (P <0.05). The highest response was obtained when DOs were first added to COCs at 3 h of IVM, after being pre-treated (0–3 h) as COCs + FSH. Compared to control, co-culture with DOs from 3 h did not affect implantation rates but more than doubled fetal yield (21 % vs 48 %; P <0.05). GDF9 Western blot analysis was unable to detect any differences in quantity or form of GDF9 (17 and 65 kDa) in extracts of DO at 0 h or 3 h. Conclusions This study provides new knowledge on means to improve oocyte quality in vitro which has the potential to significantly aid human infertility treatment and animal embryo production technologies. |
Keywords: | Oocyte in vitro maturation (IVM) GDF9 BMP15 Oocyte-secreted factor (OSFs) |
Rights: | © Springer Science+Business Media New York 2014 |
DOI: | 10.1007/s10815-013-0152-5 |
Published version: | http://dx.doi.org/10.1007/s10815-013-0152-5 |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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hdl_81765.pdf | Accepted version | 450.63 kB | Adobe PDF | View/Open |
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