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https://hdl.handle.net/2440/8147
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Type: | Journal article |
Title: | Leptin and leptin receptor expression in the rat ovary |
Author: | Ryan, N. Van Der Hoek, K. Robertson, S. Norman, R. |
Citation: | Endocrinology, 2003; 144(11):5006-5013 |
Publisher: | Endocrine Soc |
Issue Date: | 2003 |
ISSN: | 0013-7227 1945-7170 |
Statement of Responsibility: | Natalie K. Ryan, Kylie H. Van der Hoek, Sarah A. Robertson and Robert J. Norman |
Abstract: | Leptin is an important satiety hormone and reproductive regulator and is found, along with its receptors, throughout the ovary. To date, the changes in ovarian expression of both of these proteins throughout the estrous cycle has not been studied, and the examination of protein expression has not distinguished between different forms of the receptor. In this study leptin mRNA expression in the immature gonadotropin-primed rat ovary increased 3-fold after human chorionic gonadotropin administration, followed by a dramatic increase in mRNA for both the short form (Ob-Ra) and the long form (Ob-Rb) of the leptin receptor (8- and 7-fold, respectively). A corresponding increase in mRNA expression of the receptor was not observed in isolated preovulatory follicles. Using immunohistochemistry, we observed protein expression of the long form of the leptin receptor (Ob-Rb) in the ovary, with high intensities observed in oocytes and endothelial cells as well as thecal cells and corpora lutea. These results suggest that ovarian expression of leptin and its receptor is regulated across the cycle by gonadotropins, with peak expression at ovulation, indicating a possible involvement in oocyte maturation, angiogenesis, follicle rupture, or subsequent corpus luteum formation. |
Keywords: | Ovary Ovarian Follicle Animals Rats Rats, Sprague-Dawley Estradiol Progesterone Chorionic Gonadotropin Leptin Receptors, Cell Surface RNA RNA, Messenger Organ Size Culture Techniques Follicular Phase Time Factors Female Receptors, Leptin |
Description: | Copyright © 2003 by The Endocrine Society |
DOI: | 10.1210/en.2003-0584 |
Published version: | http://dx.doi.org/10.1210/en.2003-0584 |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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