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https://hdl.handle.net/2440/73926
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Type: | Journal article |
Title: | Distinct cellular fates for KP1019 and NAMI-A determined by X-ray fluorescence imaging of single cells |
Author: | Aitken, J. Antony, S. Weekley, C. Lai, B. Spiccia, L. Harris, H. |
Citation: | Metallomics: integrated biometal science, 2012; 4(10):1051-1056 |
Publisher: | Royal Society of Chemistry |
Issue Date: | 2012 |
ISSN: | 1756-5901 1756-591X |
Statement of Responsibility: | Jade B. Aitken, Sumy Antony, Claire M. Weekley, Barry Lai, Leone Spiccia and Hugh H. Harris |
Abstract: | Small molecule ruthenium complexes show great promise as anticancer pharmaceuticals, but further rational development of these as drugs is stymied by an incomplete understanding of the mechanisms that give rise to markedly different biological behaviour for structurally similar species. X-ray fluorescence imaging at two incident energies was used to reveal the intracellular distribution of Ru in single human cells treated with KP1019, showing Ru localised in both cytosol and in the nuclear region. In addition the imaging showed that treatment with KP1019 modulated Fe distribution to resemble the Ru distribution, without affecting cellular Fe content. In stark contrast, Ru could not be visualised in cells treated with NAMI-A, indicating that it was not internalised and supporting the proposition that its activity is exerted through a membrane-binding mechanism. |
Keywords: | Cell Line, Tumor Intracellular Space Humans Iron Ruthenium Compounds Organometallic Compounds Dimethyl Sulfoxide Indazoles Spectrometry, X-Ray Emission Molecular Imaging Single-Cell Analysis |
DOI: | 10.1039/c2mt20072d |
Grant ID: | http://purl.org/au-research/grants/arc/DP0985807 http://purl.org/au-research/grants/arc/DP0984722 |
Published version: | http://dx.doi.org/10.1039/c2mt20072d |
Appears in Collections: | Aurora harvest Chemistry publications Environment Institute publications IPAS publications |
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