Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/71584
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Type: | Journal article |
Title: | High-resolution genotyping of wild barley introgression lines and fine-mapping of the threshability locus thresh-1 using the Illumina GoldenGate assay |
Author: | Schmalenbach, I. March, T. Bringezu, T. Waugh, R. Pillen, K. |
Citation: | G3: Genes, Genomes, Genetics, 2011; 1(3):187-196 |
Publisher: | Genetics Society of America |
Issue Date: | 2011 |
ISSN: | 2160-1836 2160-1836 |
Statement of Responsibility: | Inga Schmalenbach, Timothy J. March, Thomas Bringezu, Robbie Waugh and Klaus Pillen |
Abstract: | Genetically well-characterized mapping populations are a key tool for rapid and precise localization of quantitative trait loci (QTL) and subsequent identification of the underlying genes. In this study, a set of 73 introgression lines (S42ILs) originating from a cross between the spring barley cultivar Scarlett (Hordeum vulgare ssp. vulgare) and the wild barley accession ISR42-8 (H. v. ssp. spontaneum) was subjected to high-resolution genotyping with an Illumina 1536-SNP array. The array enabled a precise localization of the wild barley introgressions in the elite barley background. Based on 636 informative SNPs, the S42IL set represents 87.3% of the wild barley genome, where each line contains on average 3.3% of the donor genome. Furthermore, segregating high-resolution mapping populations (S42IL-HRs) were developed for 70 S42ILs in order to facilitate QTL fine-mapping and cloning. As a case study, we used the developed genetic resources to rapidly identify and fine-map the novel locus thresh-1 on chromosome 1H that controls grain threshability. Here, the recessive wild barley allele confers a difficult to thresh phenotype, suggesting that thresh-1 played an important role during barley domestication. Using a S42IL-HR population, thresh-1 was fine-mapped within a 4.3cM interval that was predicted to contain candidate genes involved in regulation of plant cell wall composition. The set of wild barley introgression lines and derived high-resolution populations are ideal tools to speed up the process of mapping and further dissecting QTL, which ultimately clears the way for isolating the genes behind QTL effects. |
Keywords: | Wild barley introgression lines Illumina GoldenGate assay high-resolution mapping populations threshability locus thresh-1 |
Rights: | Copyright © 2011 Schmalenbach et al. This is an open access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1534/g3.111.000182 |
Published version: | http://dx.doi.org/10.1534/g3.111.000182 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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File | Description | Size | Format | |
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hdl_71584.pdf | Published version | 1.31 MB | Adobe PDF | View/Open |
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