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https://hdl.handle.net/2440/7103
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Type: | Journal article |
Title: | Human chromosomal fragile site FRA16B is an amplified AT-rich minisatellite repeat |
Author: | Yu, S. Mangelsdorf, M. Hewett, D. Hobson, L. Baker, E. Eyre, H. Lapsys, N. Le Paslier, D. Doggett, N. Sutherland, G. Richards, R. |
Citation: | Cell, 1997; 88(3):367-374 |
Publisher: | CELL PRESS |
Issue Date: | 1997 |
ISSN: | 0092-8674 1097-4172 |
Statement of Responsibility: | Yu, Sui; Mangelsdorf, Marie; Hewett, Duncan; Hobson, Lynne; Baker, Elizabeth; Eyre, Helen J; Lapsys, Naras; Le Paslier, Denis; Doggett, Norman A; Sutherland, Grant R; Richards, Robert I |
Abstract: | Fragile sites are nonstaining gaps in chromosomes induced by specific tissue culture conditions. They vary both in population frequency and in the culture conditions required for induction. Folate-sensitive fragile sites are due to expansion of p(CCG)n trinucleotide repeats; however, the relationship between sequence composition and the chemistry of induction of fragile sites is unclear. To clarify this relationship, the distamycin A-sensitive fragile site FRA16B was isolated by positional cloning and found to be an expanded 33 bp AT-rich minisatellite repeat, p(ATATA TTATATATTATATCTAATAATATATC/ATA)n (consistent with DNA sequence binding preferences of chemicals that induce its cytogenetic expression). Therefore the mutation mechanism associated with trinucleotide repeats is also a property of minisatellite repeats (variable number tandem repeats). |
Keywords: | Chromosomes, Human, Pair 16 Humans Chromosome Fragility DNA, Satellite Blotting, Southern Cloning, Molecular Polymerase Chain Reaction Gene Amplification Base Composition Base Sequence Minisatellite Repeats Polymorphism, Genetic Chromosome Fragile Sites Molecular Sequence Data |
DOI: | 10.1016/S0092-8674(00)81875-9 |
Published version: | http://dx.doi.org/10.1016/s0092-8674(00)81875-9 |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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