Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/7008
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Sulfation of chondroitin/dermatan sulfate by cystic fibrosis pancreatic duct cells is not different from control cells |
Author: | Hill, W. Harper, G. Rozaklis, T. Hopwood, J. |
Citation: | Biochemical and molecular medicine, 1997; 62(1):85-94 |
Publisher: | ACADEMIC PRESS INC ELSEVIER SCIENCE |
Issue Date: | 1997 |
ISSN: | 1077-3150 1095-5577 |
Statement of Responsibility: | Warren G. Hill, Gregory S. Harper, Tina Rozaklis, John J. Hopwood |
Abstract: | Cystic fibrosis is associated with mutations of the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-regulated plasma membrane chloride channel. Cystic fibrosis patients have been reported to possess elevated sulfation of glycoconjugates, which may contribute to the pathogenesis of the disease. Sulfation of glycosaminoglycans by a cystic fibrosis pancreatic adenocarcinoma cell line homozygous for DeltaF508 (CFPAC-1), a control pancreatic cell line (PANC-1), two CFPAC-1 cell lines transfected with the gene for CFTR (PLJ-CFTR-4.7, TR20), and a mock-transfected CFPAC-1 control (PLJ-6) was investigated. Cells were radiolabeled with [35S]sulfate and [3H]glucosamine, and glycosaminoglycans secreted into the medium after 24 and 72 h were isolated. Chondroitinase ABC digestion of chondroitin/dermatan sulfate allowed the recovery of disaccharides which were analyzed for their degree of sulfation by strong anion-exchange HPLC. No differences in the extent of sulfation by any of the cell lines were noted. However, glycoaminoglycans synthesized by cystic fibrosis cells consistently exhibited twofold higher [35S]-sulfate:[3H]glucosamine ratios than the controls. We conclude that CFTR plays no role in the sulfation of chondroitin/dermatan sulfate by pancreatic cells and that isotope incorporation ratios alone are insufficient evidence of changes in sulfation levels. |
Keywords: | cystic fibrosis CFTR sulfation glycosaminoglycans pancreatic cells |
Rights: | Copyright © 1997, Elsevier |
DOI: | 10.1006/bmme.1997.2625 |
Published version: | http://dx.doi.org/10.1006/bmme.1997.2625 |
Appears in Collections: | Aurora harvest Paediatrics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.