Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/66086
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Modified active site coordination in a clinical mutant of sulfite oxidase |
Author: | Doonan, C. Wilson, H. Rajagopalan, K. Garrett, R. Bennett, B. Prince, R. George, G. |
Citation: | Journal of the American Chemical Society, 2007; 129(30):9421-9428 |
Publisher: | Amer Chemical Soc |
Issue Date: | 2007 |
ISSN: | 0002-7863 1520-5126 |
Statement of Responsibility: | Christian J. Doonan, Heather L. Wilson, K. V. Rajagopalan, Robert M. Garrett and Brian Bennett, Roger C. Prince, and Graham N. George |
Abstract: | The molybdenum site of the Arginine 160 --> Glutamine clinical mutant of the physiologically vital enzyme sulfite oxidase has been investigated by a combination of X-ray absorption spectroscopy and density functional theory calculations. We conclude that the mutant enzyme has a six-coordinate pseudo-octahedral active site with coordination of Glutamine Oepsilon to molybdenum. This contrasts with the wild-type enzyme which is five-coordinate with approximately square-based pyramidal geometry. This difference in the structure of the molybdenum site explains many of the properties of the mutant enzyme which have previously been reported. |
Keywords: | Molybdenum Organometallic Compounds Arginine Glutamine Crystallography, X-Ray Spectrum Analysis Binding Sites Protein Conformation Mutation Hydrogen-Ion Concentration Algorithms X-Rays Computer Simulation Sulfite Oxidase |
Rights: | © 2007 American Chemical Society |
DOI: | 10.1021/ja071402a |
Published version: | http://dx.doi.org/10.1021/ja071402a |
Appears in Collections: | Aurora harvest 5 Chemistry publications Environment Institute publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.