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https://hdl.handle.net/2440/65048
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Type: | Journal article |
Title: | Dietary zinc and metallothionein on small intestinal disaccharidases activity in mice |
Author: | Tran, C. Cool, J. Xian, C. |
Citation: | World Journal of Gastroenterology, 2011; 17(3):354-360 |
Publisher: | W J G Press |
Issue Date: | 2011 |
ISSN: | 1007-9327 2219-2840 |
Statement of Responsibility: | Cuong D Tran, Johanna Cool and Cory J Xian |
Abstract: | <h4>Aim</h4>To examine the effect of increasing dietary zinc (Zn) intake and the lack of metallothionein (MT) expression on activity of small intestinal disaccharidases.<h4>Methods</h4>MT-I and II knockout (MT-/-) and wild-type (MT+/+) female mice at 3.5 wk of age were randomly fed with a diet containing 2 (2 Zn), 15 (15 Zn) or 50 (50 Zn) mg Zn/kg (n = 8/group/genotype) for 5 wk. Small intestinal segments (duodenum, jejunum and ileum) were collected and either fixed in 10% formalin for histological analysis or snap frozen in liquid nitrogen for sucrase, lactase and maltase activity analyses.<h4>Results</h4>Plasma Zn was significantly (P < 0.05) lower (33%) in MT-/- compared with MT+/+ mice fed the 2 Zn diet. Villus height and crypt depth were increased by approximately 15% in MT+/+ mice compared with MT-/- mice. Duodenal disaccharidase activities were significantly higher in MT+/+ compared with MT-/- mice particularly in those fed the 2 Zn diet. For the 50 Zn diet, jejunal sucrase and lactase activities were significantly higher in MT-/- (13 313 ± 2314; 4107 ± 364 μmol glucose/well/min/g tissue, respectively) compared with MT+/+ mice (7054 ± 608; 1818 ± 174). Similarly, ileal lactase activities were higher in MT-/- (1480 ± 192) compared with MT+/+ (629 ± 353) mice particularly those fed the 2 Zn diet.<h4>Conclusion</h4>Increasing dietary Zn has little effect on disaccharidases activity in MT wild-type mice. The presence of MT may enhance morphological and functional development of the gut. |
Keywords: | Lactase Maltase Sucrase Metallothionein Diet Zinc |
Rights: | Copyright ©2011 Baishideng Publishing Group Co., Limited. All rights reserved. |
DOI: | 10.3748/wjg.v17.i3.354 |
Published version: | http://dx.doi.org/10.3748/wjg.v17.i3.354 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 5 |
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