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https://hdl.handle.net/2440/62690
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Type: | Journal article |
Title: | Glutamate receptor-like genes form Ca²⁺ channels in pollen tubes and are regulated by pistil D-serine |
Other Titles: | Glutamate receptor-like genes form Ca2+ channels in pollen tubes and are regulated by pistil D-serine |
Author: | Michard, E. Lima, P. Borges, F. Silva, A. Portes, M. Carvalho, J. Gilliham, M. Liu, L. Obermeyer, G. Feijo, J. |
Citation: | Science, 2011; 332(6028):434-437 |
Publisher: | Amer Assoc Advancement Science |
Issue Date: | 2011 |
ISSN: | 0036-8075 1095-9203 |
Statement of Responsibility: | Erwan Michard, Pedro T. Lima, Filipe Borges, Ana Catarina Silva, Maria Teresa Portes, João E. Carvalho, Matthew Gilliham, Lai-Hua Liu, Gerhard Obermeyer and José A Feijó |
Abstract: | Elevations in cytosolic free calcium concentration ([Ca2+]cyt) constitute a fundamental signal transduction mechanism in eukaryotic cells, but the molecular identity of Ca2+ channels initiating this signal in plants is still under debate. Here, we show by pharmacology and loss-of-function mutants that in tobacco and Arabidopsis, glutamate receptor–like genes (GLRs) facilitate Ca2+ influx across the plasma membrane, modulate apical [Ca2+]cyt gradient and consequently pollen tube growth and morphogenesis. Additionally, wild-type pollen tubes grown in pistils of knock-out mutants for serine-racemase (SR1) displayed growth defects consistent with a decrease in GLR activity. Our findings reveal a novel plant signaling mechanism between male gametophyte and pistil tissue analogous to amino acid–mediated communication commonly observed in animal nervous systems. |
Keywords: | Cell Membrane Cytosol Plants, Genetically Modified Arabidopsis Tobacco Flowers Calcium 6-Cyano-7-nitroquinoxaline-2,3-dione Racemases and Epimerases Serine Glycine Calcium Channels Receptors, Glutamate Excitatory Amino Acid Agonists Excitatory Amino Acid Antagonists Patch-Clamp Techniques Calcium Signaling Gene Expression Regulation, Plant Morphogenesis Genes, Plant Pollen Tube |
Rights: | © 2011 American Association for the Advancement of Science. All Rights Reserved. |
DOI: | 10.1126/science.1201101 |
Published version: | http://www.sciencemag.org/content/332/6028/434 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 5 |
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