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https://hdl.handle.net/2440/61128
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Type: | Journal article |
Title: | In vitro and in vivo characterization of putative porcine embryonic stem cells |
Author: | Vassiliev, I. Vassilieva, S. Beebe, L. Harrison, S. McIlfatrick, S. Nottle, M. |
Citation: | Cellular Reprogramming, 2010; 12(2):223-230 |
Publisher: | Mary Ann Liebert, Inc. |
Issue Date: | 2010 |
ISSN: | 2152-4971 2152-4998 |
Statement of Responsibility: | Ivan Vassiliev, Svetlana Vassilieva, Luke F.S. Beebe, Sharon J. Harrison, Stephen M. McIlfatrick and Mark B. Nottle |
Abstract: | We have developed a new method for the isolation of porcine embryonic stem cells (ESCs) from in vivo-derived and in vitro-produced embryos. Here we describe the isolation and characterization of several ESC lines established using this method. Cells from these lines were passaged up to 14 times, during which they were repeatedly cryopreserved. During this time, ESCs maintained their morphology and continued to express Oct 4, Nanog, and SSEA1. These cells formed embryoid bodies in suspension culture, and could be directed to differentiate into various lineages representative of all three germ layers in vitro. When injected into blastocysts these cells localized in the inner cell mass of blastocysts. To examine their pluripotency further, cells were injected into host blastocysts and transferred to recipient animals. Of the six transfers undertaken, one recipient became pregnant and gave birth to a litter of one male and three female piglets. Microsatellite analysis of DNA extracted from the tail tissue of these piglets indicated that two female piglets were chimaeric. |
Keywords: | Ovary Cell Line Blastocyst Animals Swine Cryopreservation Cell Culture Techniques Cell Differentiation Cell Lineage Pregnancy Pregnancy, Animal Genotype Models, Genetic Female Embryonic Stem Cells |
Rights: | © Mary Ann Liebert, Inc. |
DOI: | 10.1089/cell.2009.0053 |
Published version: | http://dx.doi.org/10.1089/cell.2009.0053 |
Appears in Collections: | Aurora harvest 5 Obstetrics and Gynaecology publications |
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