Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/56166
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Investigation on the morphology of precipitated chemicals from TE buffer on solid substrates. |
Author: | Wang, H. Zhang, L. Zhang, F. An, H. Chen, S. Li, H. Wang, P. Wang, X. Wang, Y. Yang, H. |
Citation: | Surface Review and Letters, 2007; 14(06):1121-1128 |
Publisher: | World Scientific Publ Co Pte Ltd |
Issue Date: | 2007 |
ISSN: | 0218-625X 1793-6667 |
Statement of Responsibility: | Huabin Wang, Lijuan Zhang, Feng Zhang, Hongjie An, Shimou Chen, Hai Li, Peng Wang, Xinyang Wang, Yang Wang and Haijun Yang |
Abstract: | Buffer is often involved in the biological studies on a surface with the scanning probe microscopy and the remnant of the salts of buffer may affect the correct interpretation of the experimental results. However, the knowledge on the performance of chemicals of buffer on a substrate is still very poor. TE solution (Trisâ HCl, NaCl, and ethylene diamine tetraacetic acid (EDTA)) is a widely used buffer in stocking biological molecules. Herein, we report the performance of the precipitated chemicals from TE solution on two typical substrates with several commonly used sample preparation methods. The results showed that the chemicals in TE solution could self-organize into parallel nanofilaments on hydrophobic highly oriented pyrolitic graphite (HOPG) surface by blotting the shortly incubated solution droplet from the substrate or by drying the diluted solution droplet naturally. In contrast, no such special structures were observed on hydrophilic mica using the same methods. By imaging in TE solutions, no special structures were found on either the HOPG or the mica. Moreover, the effects of the concentration of chemicals of TE buffer on the morphology of precipitated chemicals were also investigated. |
Description: | © World Scientific Publishing Co. |
DOI: | 10.1142/S0218625X0701069X |
Published version: | http://dx.doi.org/10.1142/s0218625x0701069x |
Appears in Collections: | Aurora harvest Earth and Environmental Sciences publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.