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https://hdl.handle.net/2440/54799
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Type: | Journal article |
Title: | Interaction of dietary zinc and intracellular binding protein metallothionein in postnatal bone growth |
Author: | Fong, L. Tan, K. Tran, C. Cool, J. Scherer, M. Elovaris, R. Coyle, P. Foster, B. Rofe, A. Xian, C. |
Citation: | Bone, 2009; 44(6):1151-1162 |
Publisher: | Elsevier Science Inc |
Issue Date: | 2009 |
ISSN: | 8756-3282 1873-2763 |
Statement of Responsibility: | Laura Fong, Kim Tan, Cuong Tran, Johanna Cool, Michaela A. Scherer, Rachel Elovaris, Peter Coyle, Bruce K. Foster, Allan M. Rofe and Cory J. Xian |
Abstract: | Zinc and its binding protein, metallothionein (MT), are important in regulating growth and development, and yet it is unclear how dietary Zn and MT interact in regulating bone growth. Here, 3.5-week female MT-I&II knockout (MT(-/-)) and wild type (MT(+/+)) mice were fed diets containing 2.5 (limiting, Zn-L), 15 or 50 mg Zn/kg (Zn adequate) for 5 or 9 weeks, and effects were analysed on structure and function of growth plate and metaphysis, two structures important for bone growth. Zn limitation did not affect bone growth in MT(+/+) mice. However, MT(-/-) mice, having lower Zn concentrations in plasma and long bone, showed growth retardation as demonstrated by lower body length gain, shorter and smaller tibia/femur, lower chondrocyte proliferation, reduced metaphysis heights, but increased osteoclast densities on trabecular bone, particularly in mice fed Zn-L diet. Interestingly, mRNA expression of MT-I&II was induced in the growth plate of MT(+/+) mice fed the Zn-L diet possibly compensating for Zn limitation. Growth plate MT-III expression increased in MT(-/-) mice fed the adequate Zn diet, whereas metaphyseal MT-III was significantly upregulated in MT(-/-) mice fed Zn-L diet, possibly as a compensatory mechanism or exacerbating effects of Zn limitation. Consistent with the increased osteoclast numbers, a higher ratio of RANKL/OPG gene expression was found in bone of mutant mice fed lower Zn diets. These results indicate that interaction between dietary Zn and endogenous MT is important for maximal bone growth, and MT is particularly important in the regulation of Zn pool for bone growth during moderate Zn limitation. |
Keywords: | Growth Plate Chondrocytes Animals Mice, Knockout Mice Zinc Metallothionein Protein Isoforms Reverse Transcriptase Polymerase Chain Reaction Bone Development Dietary Supplements Female In Vitro Techniques |
Description: | Copyright © 2009 Elsevier Inc. All rights reserved. |
DOI: | 10.1016/j.bone.2009.02.011 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/525233/description#description |
Published version: | http://dx.doi.org/10.1016/j.bone.2009.02.011 |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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