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https://hdl.handle.net/2440/46755
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Type: | Journal article |
Title: | Increased biological oxidation and reduced anti-oxidant enzyme activity in pre-eclamptic placentae |
Author: | Vanderlelie, J. Venardos, K. Clifton, V. Gude, N. Clarke, F. Perkins, A. |
Citation: | Placenta, 2005; 26(1):53-58 |
Publisher: | W B Saunders Co Ltd |
Issue Date: | 2005 |
ISSN: | 0143-4004 1532-3102 |
Statement of Responsibility: | J. Vanderlelie, K. Venardos, V.L. Clifton, N.M. Gude, F.M. Clarke, A.V. Perkins |
Abstract: | Oxidative stress occurs when cellular levels of reactive oxygen species exceed anti-oxidant capabilities and has been implicated in the pathogenesis of pre-eclampsia. In this study we have examined the tissue levels of endogenous anti-oxidant proteins (superoxide dismutase, glutathione peroxidase, thioredoxin reductase and thioredoxin) and the level of lipid and protein oxidation in placental samples from normal and pre-eclamptic pregnancies. Pre-eclamptic tissue homogenates demonstrated significantly increased levels of lipid peroxidation (20.68 +/- 7.811 microM protein versus 5.33 +/- 4.03 microM/mg protein, P < 0.001) and a trended increase in protein carbonyl concentration (248.1 +/- 97.71 units/mg protein versus 209.7 +/- 82.6 U/mg protein) when compared to controls. The levels and activities of the anti-oxidant proteins superoxide dismutase (2.48 +/- 0.6 U/mg protein versus 2.02 +/- 0.51 U/mg protein, P <0.02), thioredoxin reductase (19.25 +/- 9.81 U/mg protein versus 13.02 +/- 5.66 U/mg protein,P = 0.02), thioredoxin (107.00 +/- 18.11 ng/mg protein versus 91.12 +/- 21.18 ng/mg protein, P = 0.02) and glutathione peroxidase (17.33 +/- 6.63 mmol/min/mg protein versus 11.50 +/- 3.11 mmol/min/mg, P < 0.02) were all found to be significantly reduced when comparing pre-eclamptic placental tissue homogenates to gestational age-matched control placentae from non-pre-eclamptic pregnancies. The results of this study demonstrate a decreased enzymatic anti-oxidant capacity and increased oxidation in placental tissue from pre-eclamptic women, which may contribute to the pathogenesis of this complex disorder. |
Keywords: | Placenta Humans Pre-Eclampsia Oxidoreductases Glutathione Peroxidase Superoxide Dismutase Antioxidants Oxidation-Reduction Oxidative Stress Pregnancy Adult Female Thioredoxins Thioredoxin-Disulfide Reductase |
DOI: | 10.1016/j.placenta.2004.04.002 |
Published version: | http://www.placentajournal.org/article/S0143-4004(04)00104-3/abstract |
Appears in Collections: | Aurora harvest Physiology publications |
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