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Type: Journal article
Title: Detection of novel genotypes in progeny from a controlled cross between isolates of Uncinula necator belonging to distinct phenetic groups
Author: Stummer, B.
Scott, E.
Citation: Australasian Plant Pathology, 2003; 32(2):213-218
Publisher: CSIRO Publishing
Issue Date: 2003
ISSN: 0815-3191
Abstract: Sexual recombination and segregation in 18 ascospore progeny of Uncinula necator were analysed by mating type (Mat locus), RFLP and PCR markers. Progeny were analysed from a cross of parental isolates representing the genetically distinct phenetic Groups I (AHd2) and II (BNb2). A total of 27 markers was analysed, consisting of four single loci (Mat locus and three RFLP probes), 16 multi-loci (three multi-copy fingerprinting probes) and seven PCR loci. Novel hybrid genotypes were detected in 28 % of the ascospore-derived progeny using the multi-copy RFLP probes, pUn122-11 and pUnP27, and the PCR primers (CAC)₅ and R1, confirming that recombination can occur between isolates representative of the two phenetic groups. This study provides evidence that out-crossing between isolates of U. necator generates novel genotypes and, as such, may have important implications for disease management practices, such as the durability of fungicides and host resistance.
Keywords: Grapevine powdery mildew; recombination; genetic analysis; RFLP and PCR markers
RMID: 0020030160
DOI: 10.1071/AP03002
Appears in Collections:Agriculture, Food and Wine publications

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