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https://hdl.handle.net/2440/23834
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Type: | Journal article |
Title: | Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucans |
Author: | Burton, R. Wilson, S. Hrmova, M. Harvey, A. Shirley, N. Medhurst, A. Stone, B. Newbigin, E. Bacic, A. Fincher, G. |
Citation: | Science, 2006; 311(5769):1940-1942 |
Publisher: | Amer Assoc Advancement Science |
Issue Date: | 2006 |
ISSN: | 0036-8075 1095-9203 |
Statement of Responsibility: | Rachel A. Burton, Sarah M. Wilson, Maria Hrmova, Andrew J. Harvey, Neil J. Shirley, Anne Medhurst, Bruce A. Stone, Edward J. Newbigin, Antony Bacic, Geoffrey B. Fincher |
Abstract: | A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-ß-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-ß-D-glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-ß-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-ß-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-ß-D-glucan biosynthesis. |
Keywords: | Cell Wall Plants, Genetically Modified Arabidopsis Hordeum Plant Leaves Glucosyltransferases beta-Glucans Antibodies, Monoclonal Genomics Transformation, Genetic Synteny Genes, Plant Genome, Plant Quantitative Trait Loci Oryza |
DOI: | 10.1126/science.1122975 |
Published version: | http://dx.doi.org/10.1126/science.1122975 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
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