Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/13439
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Two novel subgenomic RNAs derived from RNA3 of tomato aspermy cucumovirus |
Author: | Shi, B.J. Ding, S.W. Symons, R. |
Citation: | Journal of General Virology, 1997; 78(3):505-510 |
Publisher: | Microbiology Society |
Issue Date: | 1997 |
ISSN: | 0022-1317 1465-2099 |
Abstract: | Two abundant subgenomic RNAs, designated RNA 3B and RNA 5, were found to be associated with the V strain of tomato aspermy cucumovirus (V-TAV). Sequence determination showed that the 3'-terminal 323 nucleotides (nt) of RNA 3B was identical to RNA 5, whereas its 5'-terminal 163 nt was a direct repeat (one nt difference) of the 5'-half of RNA 5, and that both RNAs are completely homologous to the 3'-terminal untranslated region of TAV RNA 3. TAV RNAs 3B and 5 were also detected in the infection of a pseudorecombinant virus consisting of TAV RNA 3 and RNAs 1 and 2 from cucumber mosaic virus. Furthermore, only RNA 5, not RNA 3B, was detected in a TAV mutant in which one of the repeats was deleted from RNA 3. These genetic studies clearly show that both RNA species are derived from TAV RNA 3. However, in contrast to TAV RNAs 4 and 4A, which encode coat protein and 2b protein, respectively, RNAs 3B and 5 represent a novel class of subgenomic RNAs from TAV that do not function as mRNAs. Possible functional roles for such a class of viral subgenomic RNAs are discussed. |
Keywords: | Cucumovirus RNA, Viral Base Sequence Sequence Homology, Nucleic Acid Molecular Sequence Data Solanum lycopersicum |
DOI: | 10.1099/0022-1317-78-3-505 |
Published version: | http://dx.doi.org/10.1099/0022-1317-78-3-505 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.