Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/134215
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Type: | Journal article |
Title: | Plant trans-Golgi network/early endosome pH regulation requires cation chloride cotransporter (CCC1) |
Author: | McKay, D.W. McFarlane, H.E. Qu, Y. Situmorang, A. Gilliham, M. Wege, S. |
Citation: | eLife, 2022; 11:1-28 |
Publisher: | eLife Sciences Publications Ltd |
Issue Date: | 2022 |
ISSN: | 2050-084X 2050-084X |
Statement of Responsibility: | W Daniel McKay, E Heather McFarlane, Yue Qu, Apriadi Situmorang, Matthew Gilliham, Stefanie Wege |
Abstract: | Plant cells maintain a low luminal pH in the Trans-Golgi-Network/Early Endosome (TGN/EE), the organelle in which the secretory and endocytic pathways intersect. Impaired TGN/EE pH regulation translates into severe plant growth defects. The identity of the proton pump and proton/ion antiporters that regulate TGN/EE pH have been determined, but an essential component required to complete the TGN/EE membrane transport circuit remains unidentified - a pathway for cation and anion efflux. Here, we have used complementation, genetically encoded fluorescent sensors, and pharmacological treatments to demonstrate that Arabidopsis Cation Chloride Cotransporter (CCC1) is this missing component necessary for regulating TGN/EE pH and function. Loss of CCC1 function leads to alterations in TGN/EE-mediated processes including endocytic trafficking, exocytosis and response to abiotic stress, consistent with the multitude of phenotypic defects observed in ccc1 knockout plants. This discovery places CCC1 as a central component of plant cellular function. |
Keywords: | Plant biology |
Rights: | © 2022, McKay et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
DOI: | 10.7554/eLife.70701 |
Grant ID: | http://purl.org/au-research/grants/arc/DE160100804 http://purl.org/au-research/grants/arc/FT130100709 http://purl.org/au-research/grants/arc/CE140100008 http://purl.org/au-research/grants/arc/DE170100054 |
Published version: | http://dx.doi.org/10.7554/elife.70701 |
Appears in Collections: | Agriculture, Food and Wine publications |
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hdl_134215.pdf | Published Version | 14.49 MB | Adobe PDF | View/Open |
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