Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/131935
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Type: Journal article
Title: Rab-dependent vesicular traffic affects female gametophyte development in Arabidopsis
Author: Rojek, J.
Tucker, M.R.
Pinto, S.C.
Rychłowski, M.
Lichocka, M.
Soukupova, H.
Nowakowska, J.
Bohdanowicz, J.
Surmacz, G.
Gutkowska, M.
Citation: Journal of Experimental Botany, 2020; 72(2):320-340
Publisher: Oxford University Press
Issue Date: 2020
ISSN: 0022-0957
1460-2431
Statement of
Responsibility: 
Joanna Rojek, Matthew R Tucker, Sara C Pinto, Michał Rychłowski, Małgorzata Lichocka, Hana Soukupova ... et al.
Abstract: Eukaryotic cells rely on the accuracy and efficiency of vesicular traffic. In plants, disturbances in vesicular trafficking are well studied in quickly dividing root meristem cells or polar growing root hairs and pollen tubes. The development of the female gametophyte, a unique haploid reproductive structure located in the ovule, has received far less attention in studies of vesicular transport. Key molecules providing the specificity of vesicle formation and its subsequent recognition and fusion with the acceptor membrane are Rab proteins. Rabs are anchored to membranes by covalently linked geranylgeranyl group(s) that are added by the Rab geranylgeranyl transferase (RGT) enzyme. Here we show that Arabidopsis plants carrying mutations in the gene encoding the beta subunit of RGT (rgtb1) exhibit severely disrupted female gametogenesis and this effect is of sporophytic origin. Mutations in rgtb1 lead to internalization of the PIN1 and PIN3 proteins from the basal membranes to vesicles in pro-vascular cells of the funiculus. Decreased transport of auxin out of the ovule is accompanied by auxin accumulation in a tissue surrounding the growing gametophyte. In addition, female gametophyte development arrests at the uni- or binuclear stage in a significant portion of the rgtb1 ovules. These observations suggest that communication between the sporophyte and the developing female gametophyte relies on Rab dependent vesicular traffic of the PIN1 and PIN3 transporters and auxin efflux out of the ovule.
Keywords: Arabidopsis
PIN1
PIN3
Rab
Rab geranylgeranyl transferase
auxin transport
female gametophyte
funiculus
ovule
Rights: © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1093/jxb/eraa430
Grant ID: http://purl.org/au-research/grants/arc/DP180104092
Appears in Collections:Agriculture, Food and Wine publications
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