Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/131090
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | QTL mapping: an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine |
Author: | Bartle, L. Peltier, E. Sundstrom, J.F. Sumby, K. Mitchell, J.G. Jiranek, V. Marullo, P. |
Citation: | Applied Microbiology and Biotechnology, 2021; 105(12):5053-5066 |
Publisher: | Springer-Verlag |
Issue Date: | 2021 |
ISSN: | 0175-7598 1432-0614 |
Statement of Responsibility: | Louise Bartle, Emilien Peltier, Joanna F. Sundstrom, Krista Sumby, James G. Mitchell, Vladimir Jiranek, Philippe Marullo |
Abstract: | The two most commonly used wine microorganisms, Saccharomyces cerevisiae yeast and Oenococcus oeni bacteria, are responsible for completion of alcoholic and malolactic fermentation (MLF), respectively. For successful co-inoculation, S. cerevisiae and O. oeni must be able to complete fermentation; however, this relies on compatibility between yeast and bacterial strains. For the first time, quantitative trait loci (QTL) analysis was used to elucidate whether S. cerevisiae genetic makeup can play a role in the ability of O. oeni to complete MLF. Assessment of 67 progeny from a hybrid S. cerevisiae strain (SBxGN), co-inoculated with a single O. oeni strain, SB3, revealed a major QTL linked to MLF completion by O. oeni. This QTL encompassed a well-known translocation, XV-t-XVI, that results in increased SSU1 expression and is functionally linked with numerous phenotypes including lag phase duration and sulphite export and production. A reciprocal hemizygosity assay was performed to elucidate the effect of the gene SSU1 in the SBxGN background. Our results revealed a strong effect of SSU1 haploinsufficiency on O. oeni’s ability to complete malolactic fermentation during co-inoculation and pave the way for the implementation of QTL mapping projects for deciphering the genetic bases of microbial interactions. |
Keywords: | Yeast-bacteria interactions; malolactic fermentation; QTL; wine; SSU1 |
Rights: | © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 |
DOI: | 10.1007/s00253-021-11376-x |
Grant ID: | http://purl.org/au-research/grants/arc/IC170100008 |
Published version: | http://dx.doi.org/10.1007/s00253-021-11376-x |
Appears in Collections: | Agriculture, Food and Wine publications ARC Training Centre for Innovative Wine Production publications Aurora harvest 4 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_131090.pdf | Accepted version | 2.5 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.