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https://hdl.handle.net/2440/121708
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Type: | Journal article |
Title: | Dietary micronutrient supplementation for 12 days in obese male mice restores sperm oxidative stress |
Author: | Mc Pherson, N.O. Shehadeh, H. Fullston, T. Zander-Fox, D. Lane, M. |
Citation: | Nutrients, 2019; 11(9):1-18 |
Publisher: | MDPI AG |
Issue Date: | 2019 |
ISSN: | 2072-6643 2072-6643 |
Statement of Responsibility: | Nicole O. McPherson, Helana Shehadeh, Tod Fullston, Deirdre L. Zander-Fox and Michelle Lane |
Abstract: | Male obesity, which often co-presents with micronutrient deficiencies, is associated with sub-fertility. Here we investigate whether short-term dietary supplementation of micronutrients (zinc, selenium, lycopene, vitamins E and C, folic acid, and green tea extract) to obese mice for 12 days (designed to span the epididymal transit) could improve sperm quality and fetal outcomes. Five-week-old C57BL6 males were fed a control diet (CD, n = 24) or high fat diet (HFD, n = 24) for 10 weeks before allocation to the 12-day intervention of maintaining their original diets (CD, n = 12, HFD n = 12) or with micronutrient supplementation (CD + S, n = 12, HFD + S, n = 12). Measures of sperm quality (motility, morphology, capacitation, binding), sperm oxidative stress (DCFDA, MSR, and 8OHdG), early embryo development (2-cell cleavage, 8OHdG), and fetal outcomes were assessed. HFD + S males had reduced sperm intracellular reactive oxygen species (ROS) concentrations and 8OHdG lesions, which resulted in reduced 8OHdG lesions in the male pronucleus, increased 2-cell cleavage rates, and partial restoration of fetal weight similar to controls. Sub-fertility associated with male obesity may be restored with very short-term micronutrient supplementation that targets the timing of the transit of sperm through the epididymis, which is the developmental window where sperm are the most susceptible to oxidative damage. |
Keywords: | Spermatozoa Animals Mice, Inbred C57BL Mice Mice, Obese Infertility, Male Obesity Disease Models, Animal Micronutrients Sperm Motility Oxidative Stress Embryonic Development Dietary Supplements Male Diet, High-Fat |
Rights: | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
DOI: | 10.3390/nu11092196 |
Published version: | http://dx.doi.org/10.3390/nu11092196 |
Appears in Collections: | Aurora harvest 4 Genetics publications |
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