Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/11430
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Type: Journal article
Title: Pyruvate decarboxylase and anaerobic survival in Aspergillus nidulans
Author: Lockington, R.
Borlace, G.
Kelly, J.
Citation: Gene, 1997; 191(1):61-67
Publisher: ELSEVIER SCIENCE BV
Issue Date: 1997
ISSN: 0378-1119
1879-0038
Abstract: The presence of pyruvate decarboxylase activity has been demonstrated in Aspergillus nidulans, and a gene encoding a pyruvate decarboxylase has been isolated from this organism and physically characterized. The isolation of the pdcA gene in A. nidulans confirms the existence of the alcoholic fermentation pathway in this fungus, despite it being an obligate aerobic organism. Southern analysis showed that it is most probably a single copy gene. Several potential binding sites for a GATAR-binding protein were identified in the sequence just prior to the start point of transcription, and mutant alleles of the GATAR-binding protein-encoding gene, areA, affected pdcA mRNA levels in a manner that suggested that it influences pdcA expression in nitrogen repressing conditions. Other previously reported cases of AREA action are in nitrogen-limiting conditions. Interestingly, the production of ethanol was affected in a similar way by the same areA alleles, suggesting that changes in pdcA mRNA level are reflected in the changes in the level of ethanol production. The experiments presented here confirm that PDC levels are a major determinant of ethanol production under these conditions.
Keywords: Aspergillus nidulans
Pyruvate Decarboxylase
Fungal Proteins
DNA, Fungal
Blotting, Northern
Cloning, Molecular
Anaerobiosis
Amino Acid Sequence
Base Sequence
Alleles
Genes, Fungal
Molecular Sequence Data
DOI: 10.1016/S0378-1119(97)00032-2
Published version: http://dx.doi.org/10.1016/s0378-1119(97)00032-2
Appears in Collections:Aurora harvest 2
Genetics publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.