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dc.contributor.authorHofma, B.en
dc.contributor.authorWardill, H.en
dc.contributor.authorMavrangelos, C.en
dc.contributor.authorCampaniello, M.en
dc.contributor.authorDimasi, D.en
dc.contributor.authorBowen, J.en
dc.contributor.authorSmid, S.en
dc.contributor.authorBonder, C.en
dc.contributor.authorBeckett, E.en
dc.contributor.authorHughes, P.en
dc.identifier.citationPLoS ONE, 2018; 13(6):e0199394-1-e0199394-15en
dc.descriptionPublished: June 22, 2018en
dc.description.abstractBackground Inflammatory Bowel Disease (IBD) is characterized by overt inflammation of the intestine and is typically accompanied by symptoms of bloody diarrhea, abdominal pain and cramping. The Colonic Migrating Motor Complex (CMMC) directs the movement of colonic luminal contents over long distances. The tri-nitrobenzene sulphonic acid (TNBS) model of colitis causes inflammatory damage to enteric nerves, however it remains to be determined whether these changes translate to functional outcomes in CMMC activity. We aimed to visualize innate immune cell infiltration into the colon using two-photon laser scanning intra-vital microscopy, and to determine whether CMMC activity is altered in the tri-nitro benzene sulphonic (TNBS) model of colitis. Methods Epithelial barrier permeability was compared between TNBS treated and healthy control mice in-vitro and in-vivo. Innate immune activation was determined by ELISA, flow cytometry and by 2-photon intravital microscopy. The effects of TNBS treatment and IL-1β on CMMC function were determined using a specialized organ bath. Results TNBS colitis increased epithelial barrier permeability in-vitro and in-vivo. Colonic IL-1β concentrations, colonic and systemic CD11b+ cell infiltration, and the number of migrating CD11b+ cells on colonic blood vessels were all increased in TNBS treated mice relative to controls. CMMC frequency and amplitude were inhibited in the distal and mid colon of TNBS treated mice. CMMC activity was not altered by superfusion with IL-1β. Conclusions TNBS colitis damages the epithelial barrier and increases innate immune cell activation in the colon and systemically. Innate cell migration into the colon is readily identifiable by two-photon intra-vital microscopy. CMMC are inhibited by inflammation, but this is not due to direct effects of IL-1β.en
dc.description.statementofresponsibilityBen R. Hofma, Hannah R. Wardill, Chris Mavrangelos, Melissa A. Campaniello, David Dimasi, Joanne M. Bowen, Scott D. Smid, Claudine S. Bonder, Elizabeth A. Beckett, Patrick A. Hughesen
dc.publisherPublic Library of Science (PLoS)en
dc.rights© 2018 Hofma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.titleColonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitisen
dc.typeJournal articleen
pubs.library.collectionAnatomical Sciences publicationsen
Appears in Collections:Anatomical Sciences publications

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