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https://hdl.handle.net/2440/99331
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Type: | Journal article |
Title: | Oyster viperin retains direct antiviral activity and its transcription occurs via a signalling pathway involving a heat-stable haemolymph protein |
Author: | Green, T. Speck, P. Geng, L. Raftos, D. Beard, M. Helbig, K. |
Citation: | Journal of General Virology, 2015; 96(12):3587-3597 |
Publisher: | Microbiology Society |
Issue Date: | 2015 |
ISSN: | 0022-1317 1465-2099 |
Statement of Responsibility: | Timothy J. Green, Peter Speck, Lu Geng, David Raftos, Michael R. Beard and Karla J. Helbig |
Abstract: | Little is known about the response of non-model invertebrates, such as oysters, to viral infection. The vertebrate innate immune system detects virus-derived nucleic acids to trigger the type I interferon (IFN)-pathway, leading to the transcription of hundreds of IFN-stimulated genes (ISGs) that exert antiviral functions. Invertebrates were thought to lack the IFN-pathway based on the absence of IFN or ISGs encoded in model-invertebrate genomes. However, the oyster genome encodes many ISGs, including the well-described antiviral protein, viperin. In this study, we characterise oyster-viperin and show it localises to caveolin-1 and inhibits Dengue virus replication in a heterologous model. In a second set of experiments, we provide evidence that the hemolymph from poly(I:C)-injected oysters contains a heat-stable, protease-susceptible factor that induces hemocyte transcription of viperin mRNA in conjunction with upregulation of IFN-regulatory factor. Collectively, these results support the concept that oysters have antiviral systems that are homologous to the vertebrate IFN-pathway. |
Keywords: | Hemolymph Animals Dengue Virus Lipids Proteins Antiviral Agents Virus Replication Signal Transduction Gene Expression Regulation Amino Acid Sequence Molecular Sequence Data Ostreidae Caveolin 1 Hot Temperature |
Rights: | © 2015 The Authors |
DOI: | 10.1099/jgv.0.000300 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1053206 |
Published version: | http://dx.doi.org/10.1099/jgv.0.000300 |
Appears in Collections: | Aurora harvest 7 Molecular and Biomedical Science publications |
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