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https://hdl.handle.net/2440/97645
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dc.contributor.author | McDowall, M.L. | - |
dc.contributor.author | Wu, L.L. | - |
dc.contributor.author | Purdey, M. | - |
dc.contributor.author | Abell, A.D. | - |
dc.contributor.author | Goldys, E.M. | - |
dc.contributor.author | MacMillan, K.L. | - |
dc.contributor.author | Thompson, J.G. | - |
dc.contributor.author | Robker, R.L. | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Biology of Reproduction, 2016; 94(1):23 -1-23 -9 | - |
dc.identifier.issn | 0006-3363 | - |
dc.identifier.issn | 1529-7268 | - |
dc.identifier.uri | http://hdl.handle.net/2440/97645 | - |
dc.description | Published online before print December 9, 2015. | - |
dc.description.abstract | Reduced oocyte quality has been associated with poor fertility of high performance dairy cows during peak lactation, due to negative energy balance. We examined the role of non-esterified fatty acids (NEFA), known to accumulate within follicular fluid during under- and over-nutrition scenarios, in causing endoplasmic reticulum (ER) stress of in vitro maturated (IVM) cattle cumulus-oocyte complexes (COCs). NEFA concentrations were: palmitic acid (150 μM), oleic acid (200 μM) and steric acid (75 μM). Abattoir- derived COCs were randomly matured for 24h in the presence of NEFAs and/or an ER stress inhibitor, salubrinal. Total and hatched blastocyst yields were negatively impacted by NEFA treatment compared with controls, but this was reversed by salubrinal. ER stress markers, activating transcription factor 4 (Atf4) and heat shock protein 5 (Hspa5), but not Atf6, were significantly up-regulated by NEFA treatment within whole COCs, but reversed by co-incubation with salubrinal. Likewise, glucose uptake and lactate production, measured in spent medium samples, showed a similar pattern, suggesting that cumulus cell metabolism is sensitive to NEFAs via an ER-stress mediated process. In contrast, while mitochondrial DNA copy number was recovered in NEFA treated oocytes, oocyte autofluorescence of the respiratory chain co-factor, FAD, was lower following NEFA treatment of COCs and this was not reversed by salubrinal, suggesting the negative impact was via reduced mitochondrial function. These results reveal the significance of NEFA-induced ER stress on bovine COC developmental competence, revealing a potential therapeutic target for improving oocyte quality during peak lactation. | - |
dc.description.statementofresponsibility | Melanie L. Sutton-McDowall, Linda L.Y. Wu, Malcolm Purdey, Andrew D. Abell, Ewa M. Goldys, Keith L. MacMillan, Jeremy G. Thompson, and Rebecca L. Robker | - |
dc.language.iso | en | - |
dc.publisher | Society for the Study of Reproduction | - |
dc.rights | Copyright 2015 by The Society for the Study of Reproduction. | - |
dc.source.uri | http://dx.doi.org/10.1095/biolreprod.115.131862 | - |
dc.subject | Oocytes | - |
dc.subject | Animals | - |
dc.subject | Cattle | - |
dc.subject | Cinnamates | - |
dc.subject | Lactic Acid | - |
dc.subject | Thiourea | - |
dc.subject | Flavin-Adenine Dinucleotide | - |
dc.subject | Glucose | - |
dc.subject | Fatty Acids, Nonesterified | - |
dc.subject | Heat-Shock Proteins | - |
dc.subject | Embryo Culture Techniques | - |
dc.subject | Gene Dosage | - |
dc.subject | Female | - |
dc.subject | Activating Transcription Factor 4 | - |
dc.subject | Activating Transcription Factor 6 | - |
dc.subject | Cumulus Cells | - |
dc.subject | Endoplasmic Reticulum Stress | - |
dc.subject | Endoplasmic Reticulum Chaperone BiP | - |
dc.title | Non-esterified fatty acid-induced endoplasmic reticulum stress in cattle cumulus oocyte complexes alters cell metabolism and developmental competence | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1095/biolreprod.115.131862 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1077694 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1044969 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | McDowall, M.L. [0000-0002-4121-0202] | - |
dc.identifier.orcid | Purdey, M. [0000-0002-5063-8972] | - |
dc.identifier.orcid | Abell, A.D. [0000-0002-0604-2629] | - |
dc.identifier.orcid | Thompson, J.G. [0000-0003-4941-7731] | - |
dc.identifier.orcid | Robker, R.L. [0000-0002-1538-4604] | - |
Appears in Collections: | Aurora harvest 3 Medicine publications |
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