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https://hdl.handle.net/2440/95184
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dc.contributor.author | Menicanin, D. | - |
dc.contributor.author | Mrozik, K. | - |
dc.contributor.author | Wada, N. | - |
dc.contributor.author | Marino, V. | - |
dc.contributor.author | Shi, S. | - |
dc.contributor.author | Bartold, P. | - |
dc.contributor.author | Gronthos, S. | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Stem Cells and Development, 2014; 23(9):1001-1011 | - |
dc.identifier.issn | 1557-8534 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.uri | http://hdl.handle.net/2440/95184 | - |
dc.description.abstract | Primary periodontal ligament stem cells (PDLSCs) are known to possess multidifferentiation potential and exhibit an immunophenotype similar to that described for bone-marrow-derived mesenchymal stem cells. In the present study, bromo-deoxyuridine (BrdU)-labeled ovine PDLSCs implanted into immunodeficient mice survived after 8 weeks post-transplantation and exhibited the capacity to form bone/cementum-like mineralized tissue, ligament structures similar to Sharpey's fibers with an associated vasculature. To evaluate self-renewal potential, PDLSCs were recovered from harvested primary transplants 8 weeks post-transplantation that exhibit an immunophenotype and multipotential capacity comparable to primary PDLSCs. The re-derived PDLSCs isolated from primary transplants were implanted into secondary ectopic xenogeneic transplants. Histomorphological analysis demonstrated that four out of six donor re-derived PDLSC populations displayed a capacity to survive and form fibrous ligament structures and mineralized tissues associated with vasculature in vivo, although at diminished levels in comparison to primary PDLSCs. Further, the capacity for long-term survival and the potential role of PDLSCs in dental tissue regeneration were determined using an ovine preclinical periodontal defect model. Autologous ex vivo-expanded PDLSCs that were prelabeled with BrdU were seeded onto Gelfoam(®) scaffolds and then transplanted into fenestration defects surgically created in the periodontium of the second premolars. Histological assessment at 8 weeks post-implantation revealed surviving BrdU-positive PDLSCs associated with regenerated periodontium-related tissues, including cementum and bone-like structures. This is the first report to demonstrate the self-renewal capacity of PDLSCs using serial xenogeneic transplants and provides evidence of the long-term survival and tissue contribution of autologous PDLSCs in a preclinical periodontal defect model. | - |
dc.description.statementofresponsibility | Danijela Menicanin, Krzysztof Marek Mrozik, Naohisa Wada, Victor Marino, Songtao Shi, P. Mark Bartold, and Stan Gronthos | - |
dc.language.iso | en | - |
dc.publisher | Mary Ann Liebert | - |
dc.rights | © Mary Ann Liebert | - |
dc.source.uri | http://dx.doi.org/10.1089/scd.2013.0490 | - |
dc.subject | Cells, Cultured | - |
dc.subject | Multipotent Stem Cells | - |
dc.subject | Periodontal Ligament | - |
dc.subject | Animals | - |
dc.subject | Mice, Inbred NOD | - |
dc.subject | Sheep | - |
dc.subject | Mice | - |
dc.subject | Mice, SCID | - |
dc.subject | Stem Cell Transplantation | - |
dc.subject | Regeneration | - |
dc.subject | Cell Proliferation | - |
dc.subject | Cell Survival | - |
dc.subject | Time Factors | - |
dc.subject | Female | - |
dc.subject | Heterografts | - |
dc.title | Periodontal-ligament-derived stem cells exhibit the capacity for long-term survival, self-renewal, and regeneration of multiple tissue types in vivo | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1089/scd.2013.0490 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1043994 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Menicanin, D. [0000-0002-1178-2293] | - |
dc.identifier.orcid | Mrozik, K. [0000-0002-4890-8208] | - |
dc.identifier.orcid | Bartold, P. [0000-0002-5695-3877] | - |
dc.identifier.orcid | Gronthos, S. [0000-0002-6225-3084] | - |
Appears in Collections: | Aurora harvest 3 Medicine publications |
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