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https://hdl.handle.net/2440/94181
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Type: | Journal article |
Title: | Characterization and expression of the cytochrome P450 gene family in diamondback moth, Plutella xylostella (L.) |
Author: | Yu, L. Tang, W. He, W. Ma, X. Vasseur, L. Baxter, S. Yang, G. Huang, S. Song, F. You, M. |
Citation: | Scientific Reports, 2015; 5(1):8952-1-8952-9 |
Publisher: | Nature |
Issue Date: | 2015 |
ISSN: | 2045-2322 2045-2322 |
Statement of Responsibility: | Liying Yu, Weiqi Tang, Weiyi He, Xiaoli Ma, Liette Vasseur, Simon W. Baxter, Guang Yang, Shiguo Huang, Fengqin Song, Minsheng You |
Abstract: | Cytochrome P450 monooxygenases are present in almost all organisms and can play vital roles in hormone regulation, metabolism of xenobiotics and in biosynthesis or inactivation of endogenous compounds. In the present study, a genome-wide approach was used to identify and analyze the P450 gene family of diamondback moth, Plutella xylostella, a destructive worldwide pest of cruciferous crops. We identified 85 putative cytochrome P450 genes from the P. xylostella genome, including 84 functional genes and 1 pseudogene. These genes were classified into 26 families and 52 subfamilies. A phylogenetic tree constructed with three additional insect species shows extensive gene expansions of P. xylostella P450 genes from clans 3 and 4. Gene expression of cytochrome P450s was quantified across multiple developmental stages (egg, larva, pupa and adult) and tissues (head and midgut) using P. xylostella strains susceptible or resistant to insecticides chlorpyrifos and fiprinol. Expression of the lepidopteran specific CYP367s predominantly occurred in head tissue suggesting a role in either olfaction or detoxification. CYP340s with abundant transposable elements and relatively high expression in the midgut probably contribute to the detoxification of insecticides or plant toxins in P. xylostella. This study will facilitate future functional studies of the P. xylostella P450s in detoxification. |
Keywords: | Animals Lepidoptera Cytochrome P-450 Enzyme System Insecticides Phylogeny Gene Expression Regulation, Enzymologic Larva Pupa Insecticide Resistance Multigene Family Inactivation, Metabolic |
Rights: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
DOI: | 10.1038/srep08952 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1038/srep08952 |
Appears in Collections: | Aurora harvest 7 Molecular and Biomedical Science publications |
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hdl_94181.pdf | Published version | 1.7 MB | Adobe PDF | View/Open |
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