Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/88960
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Type: | Journal article |
Title: | Tyrosine phosphorylation enhances activity of pneumococcal autolysin LytA |
Author: | Standish, A. Whittall, J. Morona, R. |
Citation: | Microbiology, 2014; 160(12):2745-2754 |
Publisher: | Society for General Microbiology |
Issue Date: | 2014 |
ISSN: | 1465-2080 1465-2080 |
Statement of Responsibility: | Alistair J. Standish, Jonathan J. Whittall and Renato Morona |
Abstract: | For a long time tyrosine phosphorylation has been recognized as a crucial post translational regulatory mechanism in eukaryotes. However, only in the past decade has recognition been given to the crucial importance of bacterial tyrosine phosphorylation as an important regulatory feature of pathogenesis. This study describes the effect of tyrosine phosphorylation on the activity of a major virulence factor of the pneumococcus, the autolysin LytA, and a possible connection to the Streptococcus pneumoniae capsule synthesis regulatory proteins (CpsB, CpsC & CpsD). We show that in vitro pneumococcal tyrosine kinase, CpsD, and the protein tyrosine phosphatase, CpsB, act to phosphorylate and dephosphorylate LytA. Furthermore, this modulates LytA function in vitro with phosphorylated LytA binding more strongly to the choline analogue DEAE. A phospho-mimetic (Y264E) mutation of the LytA phosphorylation site displayed similar phenotypes as well as an enhanced dimerization capacity. Similarly, tyrosine phosphorylation increased LytA amidase activity, as evidenced by a turbidometric amidase activity assay. Similarly, when the phospho-mimetic mutation was introduced in the chromosomal lytA of S. pneumoniae, autolysis occurred earlier and at an enhanced rate. This study thus describes to our knowledge the first functional regulatory effect of tyrosine phosphorylation on a non-capsule related protein in the pneumococcus, and suggests a link between the regulation of LytA-dependent autolysis of the cell, and the biosynthesis of capsular polysaccharide. |
Keywords: | LytA Streptococcus pneumoniae Tyrosine phosphorylation |
Rights: | © 2014 The Authors |
DOI: | 10.1099/mic.0.080747-0 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1048749 |
Published version: | http://dx.doi.org/10.1099/mic.0.080747-0 |
Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
Files in This Item:
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hdl_88960.pdf | Accepted version | 565.98 kB | Adobe PDF | View/Open |
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