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https://hdl.handle.net/2440/85441
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Type: | Journal article |
Title: | Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions |
Author: | Mody, K. Mahony, D. Cavallaro, A. Stahr, F. Qiao, S. Mahony, T. Mitter, N. |
Citation: | International Journal of Pharmaceutics, 2014; 465(1-2):325-332 |
Publisher: | Elsevier |
Issue Date: | 2014 |
ISSN: | 1873-3476 1873-3476 |
Statement of Responsibility: | Karishma T. Mody, Donna Mahony, Antonino S. Cavallaro, Frances Stahr Shi Zhang Qiao, Timothy J. Mahony, Neena Mitter |
Abstract: | Amino functionalised mesoporous silica nanoparticles (AM-41) have been identified as a promising vaccine delivery material. The capacity of AM-41 to stabilise vaccine components at ambient temperature (23-27°C) was determined by adsorbing the model antigen ovalbumin (OVA) to AM-41 particles (OVA-41). The OVA-41 was successfully freeze-dried using the excipients 5% trehalose and 1% PEG8000. The immunological activity of OVA and the nanoparticle structure were maintained following two months storage at ambient temperature. The results of immunisation studies in mice with reconstituted OVA-41 demonstrated the induction of humoral and cell-meditated immune responses. The capacity of AM-41 particles to facilitate ambient storage of vaccine components without the loss of immunological potency will underpin the further development of this promising vaccine delivery platform. |
Keywords: | Mesoporous silica nanoparticles; protein; freeze-drying; excipients; stability; vaccine delivery |
Rights: | © 2014 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.ijpharm.2014.01.037 |
Published version: | http://dx.doi.org/10.1016/j.ijpharm.2014.01.037 |
Appears in Collections: | Aurora harvest 7 Chemical Engineering publications |
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