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Type: Journal article
Title: Calcium induces pro-anabolic effects on human primary osteoblasts associated with acquisition of mature osteocyte markers
Author: Welldon, K.
Findlay, D.
Evdokiou, A.
Ormsby, R.
Atkins, G.
Citation: Molecular and Cellular Endocrinology, 2013; 376(1):85-92
Publisher: Elsevier Sci Ireland Ltd
Issue Date: 2013
ISSN: 0303-7207
Statement of
Katie J. Welldon, David M. Findlay, Andreas Evdokiou, Renee T. Ormsby, Gerald J. Atkins
Abstract: Calcium, in combination with vitamin D, is an effective treatment for osteoporosis. Since bone mineralisation occurs concurrently with osteoblast to osteocyte transition, we hypothesised that calcium would stimulate this process. The effect of calcium (1.8-11.8mM) was tested on human primary osteoblast (NHBC) differentiation in vitro. Cultures were assayed for cell-associated mineral and gene expression associated with osteoblast differentiation and mineralisation. Treatment with calcium resulted in a striking dose- and time-dependent increase in cell-associated mineralisation. Calcium appeared to promote osteoblast to osteocyte differentiation, as indicated by increased expression of osteocalcin (OCN), E11, dentin matrix protein 1 (DMP1) and SOST mRNA. The expression of the osteoclast inhibitor, osteoprotegerin, was dramatically enhanced by calcium. Calcium also increased the ratio of PHEX mRNA expression relative to that of MEPE, suggesting a mechanism for the pro-anabolic effect. Consistent with this, calcium-dependent mineralisation was reversed in the presence of MEPE-ASARM peptides. This study suggests that calcium promotes osteoblast to osteocyte transition and concurrent matrix mineralisation, at least in part through the PHEX-MEPE axis.
Keywords: Human osteoblast; Calcium; Mineralisation; Gene expression; OPG/RANKL; ASARM
Rights: Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
RMID: 0020130886
DOI: 10.1016/j.mce.2013.06.013
Appears in Collections:Orthopaedics and Trauma publications

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