Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/771
Type: | Journal article |
Title: | Centrifugal recovery and dissolution of recombinant Gly-IGF-II inclusion bodies: the impact of feedrate and recentrifugation on protein yield |
Author: | Wong, H. O'Neill, B. Middelberg, A. |
Citation: | Bioseparation, 1996; 6(3):185-192 |
Publisher: | KLUWER ACADEMIC PUBL |
Issue Date: | 1996 |
ISSN: | 0923-179X |
Abstract: | The impact of centrifuge feedrate and multiple centrifuge passes on protein yield following recombinant Gly-Insulin-like Growth Factor II (Gly-IGF-II) inclusion-body dissolution has been investigated. Altering centrifuge feedrate did not significantly improve the overall protein yield following dissolution. Improved centrifuge recovery at a low feedrate was offset by poorer inclusion body paste purity. This reduced purity resulted in a significant loss of protein during inclusion-body dissolution due to proteolysis. Multiple centrifuge passes improved the inclusion-body paste purity. This resulted in a net improvement in the overall protein yield following dissolution. This work demonstrates that a strong interaction exists between centrifuge performance and inclusion-body dissolution for protease-sensitive products such as Gly-IGF-II. |
Keywords: | Inclusion Bodies Humans Escherichia coli Insulin-Like Growth Factor II Recombinant Proteins Centrifugation Bioreactors Biotechnology Fermentation Evaluation Studies as Topic |
Appears in Collections: | Aurora harvest 6 Chemical Engineering publications |
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