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https://hdl.handle.net/2440/75131
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Type: | Journal article |
Title: | A Combination of Local Inflammation and Central Memory T Cells Potentiates Immunotherapy in the Skin |
Author: | Fiorenza, S. Kenna, T. Comerford, I. McColl, S. Steptoe, R. Leggatt, G. Frazer, I. |
Citation: | Journal of Immunology, 2012; 189(12):5622-5631 |
Publisher: | Amer Assoc Immunologists |
Issue Date: | 2012 |
ISSN: | 0022-1767 1550-6606 |
Statement of Responsibility: | Salvatore Fiorenza, Tony J. Kenna, Iain Comerford, Shaun McColl, Raymond J. Steptoe, Graham R. Leggatt, and Ian H. Frazer |
Abstract: | Adoptive T cell therapy uses the specificity of the adaptive immune system to target cancer and virally infected cells. Yet the mechanism and means by which to enhance T cell function are incompletely described, especially in the skin. In this study, we use a murine model of immunotherapy to optimize cell-mediated immunity in the skin. We show that in vitro–derived central but not effector memory-like T cells bring about rapid regression of skin-expressing cognate Ag as a transgene in keratinocytes. Local inflammation induced by the TLR7 receptor agonist imiquimod subtly yet reproducibly decreases time to skin graft rejection elicited by central but not effector memory T cells in an immunodeficient mouse model. Local CCL4, a chemokine liberated by TLR7 agonism, similarly enhances central memory T cell function. In this model, IL-2 facilitates the development in vivo of effector function from central memory but not effector memory T cells. In a model of T cell tolerogenesis, we further show that adoptively transferred central but not effector memory T cells can give rise to successful cutaneous immunity, which is dependent on a local inflammatory cue in the target tissue at the time of adoptive T cell transfer. Thus, adoptive T cell therapy efficacy can be enhanced if CD8+ T cells with a central memory T cell phenotype are transferred, and IL-2 is present with contemporaneous local inflammation. |
Keywords: | Epidermis T-Lymphocyte Subsets Cells, Cultured Skin Animals Mice, Inbred C57BL Mice, Transgenic Mice, Knockout Mice Inflammation Adoptive Transfer Skin Transplantation Cell Communication Immune Tolerance Immunity, Cellular Immunologic Memory Epidermal Cells |
Rights: | Copyright © 2012 by The American Association of Immunologists, Inc |
DOI: | 10.4049/jimmunol.1200709 |
Published version: | http://dx.doi.org/10.4049/jimmunol.1200709 |
Appears in Collections: | Aurora harvest 4 IPAS publications Molecular and Biomedical Science publications |
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