Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/6813
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Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Chai, F. | - |
dc.contributor.author | TruongTran, A. | - |
dc.contributor.author | Evdokiou, A. | - |
dc.contributor.author | Young, G. | - |
dc.contributor.author | Zalewski, P. | - |
dc.date.issued | 2000 | - |
dc.identifier.citation | Journal of Infectious Diseases, 2000; 182(Suppl 1):S85-S92 | - |
dc.identifier.issn | 0022-1899 | - |
dc.identifier.issn | 1537-6613 | - |
dc.identifier.uri | http://hdl.handle.net/2440/6813 | - |
dc.description.abstract | To better understand the mechanisms by which zinc deficiency induces epithelial cell death, studies were done of the effects of intracellular zinc depletion induced by the zinc chelator TPEN on apoptosis-related events in human malignant epithelial cell lines LIM1215 (colonic), NCI-H292 (bronchial), and A549 (alveolar type II). In TPEN-treated cells, depletion of zinc was followed by activation of caspase-3 (as demonstrated by enzymatic assay and Western blotting), DNA fragmentation, and morphologic changes. Increase in caspase-3 activity began 1–2 h after addition of TPEN, suggesting that zinc may suppress a step just before the activation of this caspase. Caspase-6, a mediator of caspase-3 processing, also increased, but later than caspase-3. Effects of TPEN on apoptosis were completely prevented by exogenous ZnSO4 and partially prevented by peptide caspase inhibitors. A critical substrate of caspase-3 may be the cell cycle regulator p21Waf1/Cip1, which was rapidly cleaved in TPEN-treated cells to a 15-kDa fragment before further degradation. | - |
dc.description.statementofresponsibility | F. Chai, A. Q. Truong-Tran, A. Evdokiou, G. P. Young and P. D. Zalewski | - |
dc.language.iso | en | - |
dc.publisher | University of Chicago Press | - |
dc.rights | © 2000 by the Infectious Diseases Society of America. All rights reserved. | - |
dc.source.uri | http://dx.doi.org/10.1086/315914 | - |
dc.subject | Tumor Cells, Cultured | - |
dc.subject | Epithelial Cells | - |
dc.subject | Humans | - |
dc.subject | Zinc | - |
dc.subject | Ethylenediamines | - |
dc.subject | Caspases | - |
dc.subject | Cyclins | - |
dc.subject | Chelating Agents | - |
dc.subject | Enzyme Inhibitors | - |
dc.subject | Cysteine Proteinase Inhibitors | - |
dc.subject | Apoptosis | - |
dc.subject | DNA Fragmentation | - |
dc.subject | Kinetics | - |
dc.subject | Cyclin-Dependent Kinase Inhibitor p21 | - |
dc.subject | Caspase 3 | - |
dc.subject | Caspase 6 | - |
dc.title | Intracellular zinc depletion induces caspase activation and p21Waf1/Cip1 cleavage in human epithelial cell lines | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1086/315914 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Evdokiou, A. [0000-0001-8321-9806] | - |
dc.identifier.orcid | Zalewski, P. [0000-0001-5196-2611] | - |
Appears in Collections: | Aurora harvest 5 Orthopaedics and Trauma publications |
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hdl_6813.pdf | 803.49 kB | Publisher's PDF | View/Open |
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