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Type: Journal article
Title: Enhanced expression of osteocalcin mRNA in human osteoarthritic trabecular bone of the proximal femur is associated with decreased expression of interleukin-6 and interleukin-11 mRNA
Author: Findlay, D.
Atkins, G.
Forwood, M.
Kuliwaba, J.
Fazzalari, N.
Citation: Journal of Bone and Mineral Research, 2000; 15(2):332-341
Publisher: Amer Soc Bone & Mineral Res
Issue Date: 2000
ISSN: 0884-0431
Abstract: Few studies have investigated the factors or mechanisms that may lead to structural changes in OA bone. This study examines the in vivo expression of messenger RNA encoding the osteoclastogenic cytokines interleukin-6 (IL-6) and interleukin-11 (IL-11), together with the osteoblastic marker osteocalcin (OCN) and the calcitonin receptor (CTR), which in bone is exclusively expressed by osteoclasts. Total RNA was isolated from intertrochanteric trabecular bone from OA patients, and from controls taken at autopsy. The patterns of mRNA expression of IL-6, IL-11, OCN, and CTR were examined using reverse-transcription polymerase chain reaction (RT-PCR) by determining the relative ratios of the amplified products with respect to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Both IL-6 and IL-11 mRNA were significantly less abundant in OA than in the control group. Expression of IL-11 mRNA decreased significantly with age for both groups. OCN mRNA expression was significantly more abundant in OA, and there was no significant difference for CTR mRNA between the two groups. For both OCN and CTR in OA, expression increased significantly with increasing age. These differences in expression between the OA and control groups are consistent with an hypothesis that biochemical and genetic factors in bone can contribute or perhaps underlie the degenerative joint changes seen in OA.
Keywords: Femur; Humans; Osteoarthritis; Osteocalcin; RNA, Messenger; Interleukin-6; Interleukin-11; Polymerase Chain Reaction; Down-Regulation; Up-Regulation; Aged; Aged, 80 and over; Middle Aged; Female; Male
RMID: 0001001032
DOI: 10.1359/jbmr.2000.15.2.332
Appears in Collections:Orthopaedics and Trauma publications

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