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https://hdl.handle.net/2440/6775
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Type: | Journal article |
Title: | The osteoclastogenic molecules RANKL and RANK are associated with periprosthetic osteolysis |
Author: | Haynes, D. Crotti, T. Potter, A. Loric, M. Atkins, G. Howie, D. Findlay, D. |
Citation: | Journal of Bone and Joint Surgery: British Volume, 2001; 83B(6):902-911 |
Publisher: | British Editorial Soc Bone Joint Surgery |
Issue Date: | 2001 |
ISSN: | 0301-620X 2044-5377 |
Statement of Responsibility: | D. R. Haynes, T. N. Crotti, A. E. Potter, M. Loric, G. J. Atkins, D. W. Howie, and D. M. Findlay |
Abstract: | Extensive osteolysis adjacent to implants is often associated with wear particles of prosthetic material. We have investigated if RANKL, also known as osteoprotegerin ligand, osteoclast differentiation factor or TRANCE, and its natural inhibitor, osteoprotegerin (OPG), may be important in controlling this bone loss. Cells isolated from periprosthetic tissues containing wear particles expressed mRNA encoding for the pro-osteoclastogenic molecules, RANKL, its receptor RANK, monocyte colony-stimulating factor (M-CSF), interleukin (IL)-1ß, tumour necrosis factor (TNF), IL-6, and soluble IL-6 receptor, as well as OPG. Osteoclasts formed from cells isolated from periprosthetic tissues in the presence and absence of human osteoblastic cells. When osteoclasts formed in the absence of osteoblastic cells, markedly higher levels of RANKL mRNA relative to OPG mRNA were expressed. Particles of prosthetic materials also stimulated human monocytes to express osteoclastogenic molecules in vitro. Our results suggest that ingestion of prosthetic wear particles by macrophages results in expression of osteoclast-differentiating molecules and the stimulation of macrophage differentiation into osteoclasts. |
Keywords: | Cells, Cultured Macrophages Osteoclasts Osteolysis Glycoproteins Tumor Necrosis Factor-alpha Carrier Proteins Macrophage Colony-Stimulating Factor Membrane Glycoproteins Receptors, Tumor Necrosis Factor Receptors, Cytoplasmic and Nuclear RNA, Messenger Interleukin-6 Cytokines Reverse Transcriptase Polymerase Chain Reaction RANK Ligand Receptor Activator of Nuclear Factor-kappa B Osteoprotegerin |
Rights: | Copyright © 2001 by British Editorial Society of Bone and Joint Surgery |
DOI: | 10.1302/0301-620X.83B6.10905 |
Published version: | http://www.jbjs.org.uk/cgi/content/abstract/83-B/6/902 |
Appears in Collections: | Aurora harvest 5 Orthopaedics and Trauma publications Pathology publications |
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