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https://hdl.handle.net/2440/6689
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Type: | Journal article |
Title: | Regulation of osteopontin expression by type 1 collagen in preosteoblastic UMR201 cells |
Author: | Traianedes, K. Martin, T. Findlay, D. |
Citation: | Connective Tissue Research, 1996; 34(1):63-74 |
Publisher: | Informa UK Limited |
Issue Date: | 1996 |
ISSN: | 0300-8207 1607-8438 |
Abstract: | When UMR201 cells, phenotypically preosteoblastic, were placed onto a type I collagen gel, expression of osteopontin (OP) mRNA and protein were strongly upregulated, compared to cells plated onto plastic. This upregulation was dose-dependent, with respect to the concentration of collagen gel, and was observable within hours of cells having attached and spread on the substrate. Retinoic acid (RA) acted synergistically with type I collagen at each concentration to induce a much greater increase in OP mRNA than in cells on plastic. In addition, RA increased the phosphorylation of secreted OP. The exogenous collagen substrate inhibited the growth of UMR201 cells, with the extent and duration of inhibition dependent on the collagen concentration. The effect of type I collagen was specific; plating cells on fibronectin, laminin or vitronectin did not upregulate OP expression. In contrast to the effects on OP expression, the strong RA induction of alkaline phosphatase (ALP) mRNA in cells on plastic was attenuated in cells plated on type I collagen. Growth on type I collagen did not change OP mRNA stability or transcription rate, although there was decreased stability of the ALP mRNA in cells on collagen. |
Keywords: | Cell Line Osteoblasts Animals Humans Rats Ascorbic Acid Tretinoin Collagen Alkaline Phosphatase Glyceraldehyde-3-Phosphate Dehydrogenases Sialoglycoproteins Vitronectin Fibronectins Laminin Receptors, Retinoic Acid Cell Adhesion Cell Division Transcription, Genetic Gene Expression Regulation Osteopontin |
DOI: | 10.3109/03008209609028894 |
Published version: | http://dx.doi.org/10.3109/03008209609028894 |
Appears in Collections: | Aurora harvest 5 Orthopaedics and Trauma publications |
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