Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/6657
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Type: Journal article
Title: Factors regulating osteoclast formation in human tissues adjacent to peri-implant bone loss: expression of receptor activator NF[kappa]B, RANK ligand and osteoprotegerin
Author: Crotti, T.
Smith, M.
Findlay, D.
Zreiqat, H.
Ahern, M.
Weedon, H.
Hatzinikolous, G.
Capone, M.
Holding, C.
Haynes, D.
Citation: Biomaterials, 2004; 25(4):565-573
Publisher: Elsevier Sci Ltd
Issue Date: 2004
ISSN: 0142-9612
1878-5905
Statement of
Responsibility: 
T. N. Crotti, M. D. Smith, D. M. Findlay, H. Zreiqat, M. J. Ahern, H. Weedon, G. Hatzinikolous, M. Capone, C. Holding and D. R. Haynes
Abstract: Aseptic bone loss adjacent to orthopedic joint implants is a common cause of joint implant failure in humans. This study investigates the expression of key regulators of osteoclast formation, receptor activator NFκB (RANK), Receptor activator of NFκB ligand (RANKL) and osteoprotegerin (OPG), in the peri-implant tissues of patients with osteolysis compared with levels in synovial tissues from osteoarthritic and healthy subjects. Immunohistochemical studies demonstrated that significantly higher levels of RANKL protein (p<0.05) were found in the peri-implant tissues of patients with implant failure than in similar tissues from osteoarthritic and healthy subjects. In contrast, OPG protein levels were similar in all tissues. RANKL, expressed as mRNA and protein, was predominantly associated with cells containing wear particles. Dual labeling studies showed that the cells expressing RANKL protein were macrophages. In situ hybridization studies confirmed that mRNA encoding for these proteins is also expressed by cells in the peri-implant tissues. In addition, RANK mRNA was expressed in cells that contained wear particles. These findings show that abnormally high levels of RANKL are expressed in peri-implant tissues of patients with prosthetic loosening and that these abnormal levels of RANKL may significantly contribute to aseptic implant loosening.
Keywords: Osteolysis; Joint replacement; Pathology; Immunochemistry; In situ hybridization
Rights: © 2003 Elsevier Ltd.
RMID: 0020041201
DOI: 10.1016/S0142-9612(03)00556-8
Description (link): http://www.elsevier.com/wps/find/journaldescription.cws_home/30392/description#description
Appears in Collections:Orthopaedics and Trauma publications

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