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https://hdl.handle.net/2440/45571
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Type: | Journal article |
Title: | Sensing minute changes in biological cell monolayers with THz differential time-domain spectroscopy |
Author: | Liu, H. Plopper, G. Earley, S. Chen, Y. Ferguson, B. Zhang, X. |
Citation: | Biosensors and Bioelectronics, 2007; 22(6):1075-1080 |
Publisher: | Elsevier Advanced Technology |
Issue Date: | 2007 |
ISSN: | 0956-5663 1873-4235 |
Statement of Responsibility: | Hai-Bo Liu, George Plopper, Sarah Earley, Yunqing Chen, Bradley Ferguson, and X.-C. Zhang |
Abstract: | We used terahertz differential time-domain spectroscopy (THz-DTDS) to measure minute changes of bovine lung microvessel endothelial cells (BLMVEC) in response to vascular endothelial growth factor (VEGF). These changes were reflected by alterations in THz wave attenuations and THz dielectric properties of the treated cells. The VEGF-induced THz attenuations of cell monolayers correlated well with changes in transendothelial resistance, as measured using electric cell-substrate impedance sensing (ECIS). However, the morphological differences that gave rise to these changes were not observed with standard optical phase contrast microscopy. We conclude that THz-DTDS is a highly sensitive, non-invasive, powerful new tool to measure minute changes in the morphology of live, cultured cell monolayers. This method enables spectroscopic investigations of cells in the THz band, providing information unavailable through other conventional methods such as optical phase contrast microscopy and ECIS. |
Keywords: | Cells, Cultured Endothelial Cells Animals Cattle Vascular Endothelial Growth Factor A Spectrum Analysis Cell Culture Techniques Equipment Design Equipment Failure Analysis Cell Size Microwaves |
DOI: | 10.1016/j.bios.2006.02.021 |
Published version: | http://dx.doi.org/10.1016/j.bios.2006.02.021 |
Appears in Collections: | Aurora harvest Electrical and Electronic Engineering publications |
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