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https://hdl.handle.net/2440/3098
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Type: | Journal article |
Title: | Ring finger protein ZIN interacts with Human Immunodeficiency Virus Type 1 Vif |
Author: | Feng, F. Davis, A. Lake, J. Carr, J. Xia, W. Burrell, C. Li, P. |
Citation: | Journal of Virology, 2004; 78(19):10574-10581 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2004 |
ISSN: | 0022-538X 1098-5514 |
Statement of Responsibility: | Feng Feng, Adam Davis, Julie-Anne Lake, Jill Carr, Wei Xia, Christopher Burrell, and Peng Li |
Abstract: | Virion infectivity factor (Vif) protein of human immunodeficiency virus type 1 (HIV-1) is essential for the productive infection of primary human CD4 T lymphocytes and macrophages. Vif overcomes the HIV-inhibitory effects of cellular factor APOBEC3G, which has cytidine deaminase activity. We previously reported the isolation of a Vif-interacting ring finger protein, Triad 3, from a human leukocyte cDNA library, using the yeast two-hybrid system. The full-length cellular protein homologue of Triad 3 has been recently identified as the zinc finger protein inhibiting NF-B (ZIN). Sequence analysis indicates that Triad 3 protein contains all four major ring-like motifs of ZIN. We report here that ZIN binds to purified Vif in vitro and that Triad 3/ZIN interacts with HIV-1 Vif in transfected human 293T cells, as demonstrated by coimmunoprecipitation. To test the biological relevance of this interaction, we produced infectious HIV-1 NL4.3 in the presence or absence of cotransfected ZIN. HIV-1 NL4.3 virus stocks produced in the presence of exogenously expressed ZIN were twofold less infectious in a single-cycle infectivity assay than virus produced in the absence of exogenous ZIN. It was further shown that cells infected with HIV NL4.3 virus stocks produced in the presence of exogenously expressed ZIN were impaired in viral DNA synthesis by twofold. The impairment in viral reverse transcription and the reduction in single-cycle viral infectivity were both shown to be dependent on the presence of Vif in the virus producer cells. The possible mechanisms by which ZIN interferes with the early events of HIV-1 replication are discussed. |
Keywords: | Cell Line Humans HIV-1 Ubiquitin-Protein Ligases Intracellular Signaling Peptides and Proteins Carrier Proteins Gene Products, vif DNA, Viral RNA, Messenger Blotting, Western Precipitin Tests Virus Replication Transcription, Genetic Gene Expression Regulation Gene Deletion Amino Acid Sequence Zinc Fingers Protein Binding Molecular Sequence Data vif Gene Products, Human Immunodeficiency Virus |
Description: | Copyright © 2004, American Society for Microbiology. |
DOI: | 10.1128/JVI.78.19.10574-10581.2004 |
Published version: | http://jvi.asm.org/cgi/content/abstract/78/19/10574 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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