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https://hdl.handle.net/2440/30500
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Type: | Book chapter |
Title: | Immunohistochemical localization of relaxin-like factor/insulin-like peptide-3 in the bovine corpus luteum |
Author: | Nichols, N. Binta, H. Fields, P. Drost, M. Chang, S. Ivell, R. Fields, M. |
Citation: | Relaxin and related peptides, 2005 / Sherwood, O., Fields, P., Steinetz, B. (ed./s), vol.1041, pp.506-509 |
Publisher: | New York Academy of Sciences |
Publisher Place: | New York, USA |
Issue Date: | 2005 |
Series/Report no.: | Annals of the New York Academy of Sciences, Vol. 1041 |
ISBN: | 1573314854 |
Editor: | Sherwood, O. Fields, P. Steinetz, B. |
Abstract: | Relaxin-like factor/insulin-like peptide (INSL)-3 is highly expressed in the bovine corpus luteum throughout the estrous cycle and pregnancy. Demonstration of translation of the relaxin-like factor message was previously shown for the follicle but not the corpus luteum. In this study, relaxin-like factor mRNA was highly expressed in the corpus luteum on days 7 and 14 of pregnancy. Tissues were fixed in 10% neutral buffered formalin, and utilizing two different antibodies to relaxin-like factor, W3 rabbit anti-bovine and 2-8F mouse anti-bovine, relaxin-like factor was localized in fibroblast-like cells. Staining was also observed in the Leydig cells of bovine testicular tissue. No staining was observed in small and large steroidogenic luteal cells, indicating a nonsteroidogenic source of luteal relaxin-like factor. Definitive cell type identification is currently being determined via electron microscopy. |
Keywords: | Corpus Luteum Animals Cattle Insulin Proteins RNA, Messenger Immunohistochemistry Female |
DOI: | 10.1196/annals.1282.075 |
Published version: | http://dx.doi.org/10.1196/annals.1282.075 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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