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https://hdl.handle.net/2440/2993
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Type: | Journal article |
Title: | Differential expression of the mechanosensitive potassium channel TREK-1 in epicardial and endocardial myocytes in rat ventricle |
Author: | Tan, J. Liu, W. Saint, D. |
Citation: | Experimental Physiology, 2004; 89(3):237-242 |
Publisher: | Blackwell Publishing Ltd |
Issue Date: | 2004 |
ISSN: | 0958-0670 1469-445X |
Statement of Responsibility: | Joy H. C. Tan, Weihong Liu and David A. Saint |
Abstract: | Mechanoelectric feedback (MEF) is the process by which mechanical forces on the myocardium induce electrical responses. It is thought that MEF is important in controlling the beat to beat force of contraction in the ventricle, in response to fluctuations in load, and it may also play a role in controlling the dispersion of repolarization. The transduction mechanism for MEF is via stretch sensitive ion channels in the surface membrane of myocytes. Two types of stretch sensitive channels have been described; a non-selective cation channel, and a potassium selective channel. TREK-1 is a member of the recently cloned tandem pore potassium channels that has been shown to be mechanosensitive and to be expressed in rat heart. Here we report that the gene expression level of TREK-1, quantified using real-time RT-PCR against glyceraldehyde phosphate dehydrogenase (GAPDH) as a comparator gene, was found to be 0.34 ± 0.14 in endocardial cells compared to 0.02 ± 0.02 in epicardial cells (P< 0.05). To confirm that this is reflected in a different current density, whole cell TREK-1 currents, activated by chloroform, were recorded with patch clamp techniques in epicardial and endocardial cells. TREK-1 current density in epicardial and endocardial cells was 0.21 ± 0.06 pA/pF and 0.8 ± 0.27 pA/pF, respectively (P≤ 0.05). We discuss the implications of this differential expression of TREK-1 for controlling action potential repolarization when the myocardium is stretched. We hypothesize that the gene expression of TREK-1 is controlled by the different amounts of stretch experienced by muscle cells across the ventricular wall. |
Keywords: | Endocardium Heart Ventricles Pericardium Myocytes, Cardiac Animals Rats Rats, Sprague-Dawley Potassium Channels, Tandem Pore Domain Gene Expression Regulation |
Description: | The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1113/expphysiol.2003.027052 |
Published version: | http://www.blackwell-synergy.com/doi/abs/10.1113/expphysiol.2003.027052 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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