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https://hdl.handle.net/2440/27992
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Type: | Journal article |
Title: | Cloning and characterisation of polymorphic heterochromatic segments of Brachycome dichromosomatica |
Author: | Houben, A. Wanner, G. Hanson, L. Verlin, D. Leach, C. Timmis, J. |
Citation: | Chromosoma: biology of the nucleus, 2000; 109(3):206-213 |
Publisher: | Springer |
Issue Date: | 2000 |
ISSN: | 0009-5915 1432-0886 |
Statement of Responsibility: | Houben, Andreas ; Wanner, Gerhard ; Hanson, Lynda ; Verlin, Dawn ; Leach, Carolyn ; Timmis, Jeremy |
Abstract: | After selective enrichment and differential hybridisation of Cot-1 DNA fractions of plants with and without polymorphic heterochromatic segments, a repetitive sequence (called Bds1) specific to the polymorphic chromosome segments of Brachycome dichromosomatica (Brachyscome dichromosomatica) was isolated. A single repeat unit of Bds1 is 92 bp long and is organised in tandem arrays at three different polymorphic segment sites on the chromosomes of cytodeme A2. Although all three sites showed extensive polymorphism between plants, the karyotypes of all analysed mitotic root cells were stable within a single plant. Electron microscopy revealed heavily condensed chromatin structures at the most obvious polymorphic site. The mechanisms that generate and maintain the observed chromosome structure polymorphisms are discussed. |
Keywords: | Heterochromatin Membrane Transport Proteins Cation Transport Proteins Fungal Proteins Saccharomyces cerevisiae Proteins DNA, Plant DNA Primers Blotting, Southern Cytogenetic Analysis In Situ Hybridization, Fluorescence Restriction Mapping Cloning, Molecular Polymerase Chain Reaction Sequence Analysis, DNA Metaphase Base Sequence Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Gene Dosage Polymorphism, Genetic Molecular Sequence Data |
DOI: | 10.1007/s004120000105 |
Published version: | http://dx.doi.org/10.1007/s004120050430 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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