Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/27325
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Type: Journal article
Title: Microfibril-associated glycoprotein-1 (MAGP-1) is specifically located on the beads of the beaded-filament structure for fibrillin-containing microfibrils as visualized by the rotary shadowing technique
Author: Henderson, M.
Polewski, R.
Fanning, J.
Gibson, M.
Citation: Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 1996; 44(12):1389-1397
Publisher: Histochemical Society
Issue Date: 1996
ISSN: 0022-1554
1551-5044
Statement of
Responsibility: 
M Henderson, R Polewski, J C Fanning and M A Gibson
Abstract: This study used immunoelectron microscopic techniques to define the ultrastructural location of MAGP-1 on the fibrillin-containing microfibrils of the ocular zonule. A specific anti-MAGP-1 monoclonal antibody (MAb), 11B, was produced that did not crossreact with fibrillin-1 or other microfibrillar proteins. MAb 11B was shown by immunofluorescence to localize intensely to zonular tissue. Postembedding immunoelectron microscopy showed that MAGP-1 was associated with microfibrils throughout the zonule, with the exception of a narrow band of microfibrils at the junction with the lens capsule. With preembedding labeling, the anti-MAGP-1 MAb was found to localize in a crossbanding pattern, at intervals of about 50 nm, to microfibrils throughout the zonule and along bundles of microfibrils in surrounding vitreous tissue. Rotary shadowing of isolated microfibrils showed a "beads on a string" morphology with a periodicity of about 50 nm. With immunogold labeling, the anti-MAGP-1 antibody specifically localized on the beads in a symmetrical manner. Occasionally two gold partides were attached to the same bead, suggesting that multiple MAGP-1 molecules were present in the structure. The results indicate that MAGP-1 is intimately and regularly associated with the bead regions of fibrillin-containing microfibrils. The findings are consistent with a major structural role for MAGP-1 in microfibril biology.
Keywords: Eye; Animals; Cattle; Microfilament Proteins; Contractile Proteins; Antibodies, Monoclonal; Microscopy, Electron; Fluorescent Antibody Technique; Antibody Specificity; Fibrillins
Rights: ©1996 by The Histochemical Society, Inc.
RMID: 0030002695
DOI: 10.1177/44.12.8985131
Appears in Collections:Adelaide Microscopy publications

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