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https://hdl.handle.net/2440/27325
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dc.contributor.author | Henderson, M. | - |
dc.contributor.author | Polewski, R. | - |
dc.contributor.author | Fanning, J. | - |
dc.contributor.author | Gibson, M. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 1996; 44(12):1389-1397 | - |
dc.identifier.issn | 0022-1554 | - |
dc.identifier.issn | 1551-5044 | - |
dc.identifier.uri | http://hdl.handle.net/2440/27325 | - |
dc.description.abstract | This study used immunoelectron microscopic techniques to define the ultrastructural location of MAGP-1 on the fibrillin-containing microfibrils of the ocular zonule. A specific anti-MAGP-1 monoclonal antibody (MAb), 11B, was produced that did not crossreact with fibrillin-1 or other microfibrillar proteins. MAb 11B was shown by immunofluorescence to localize intensely to zonular tissue. Postembedding immunoelectron microscopy showed that MAGP-1 was associated with microfibrils throughout the zonule, with the exception of a narrow band of microfibrils at the junction with the lens capsule. With preembedding labeling, the anti-MAGP-1 MAb was found to localize in a crossbanding pattern, at intervals of about 50 nm, to microfibrils throughout the zonule and along bundles of microfibrils in surrounding vitreous tissue. Rotary shadowing of isolated microfibrils showed a "beads on a string" morphology with a periodicity of about 50 nm. With immunogold labeling, the anti-MAGP-1 antibody specifically localized on the beads in a symmetrical manner. Occasionally two gold partides were attached to the same bead, suggesting that multiple MAGP-1 molecules were present in the structure. The results indicate that MAGP-1 is intimately and regularly associated with the bead regions of fibrillin-containing microfibrils. The findings are consistent with a major structural role for MAGP-1 in microfibril biology. | - |
dc.description.statementofresponsibility | M Henderson, R Polewski, J C Fanning and M A Gibson | - |
dc.language.iso | en | - |
dc.publisher | Histochemical Society | - |
dc.rights | ©1996 by The Histochemical Society, Inc. | - |
dc.source.uri | http://dx.doi.org/10.1177/44.12.8985131 | - |
dc.subject | Eye | - |
dc.subject | Animals | - |
dc.subject | Cattle | - |
dc.subject | Microfilament Proteins | - |
dc.subject | Contractile Proteins | - |
dc.subject | Antibodies, Monoclonal | - |
dc.subject | Microscopy, Electron | - |
dc.subject | Fluorescent Antibody Technique | - |
dc.subject | Antibody Specificity | - |
dc.subject | Fibrillins | - |
dc.title | Microfibril-associated glycoprotein-1 (MAGP-1) is specifically located on the beads of the beaded-filament structure for fibrillin-containing microfibrils as visualized by the rotary shadowing technique | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1177/44.12.8985131 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Adelaide Microscopy publications Aurora harvest 2 |
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