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https://hdl.handle.net/2440/17971
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Type: | Journal article |
Title: | Host-defence skin peptides of the Australian common froglet Crinia signifera: sequence determination using positive and negative ion electrospray mass spectra |
Author: | Maselli, V. Brinkworth, C. Bowie, J. Tyler, M. |
Citation: | Rapid Communications in Mass Spectrometry, 2004; 18(18):2155-2161 |
Publisher: | John Wiley & Sons Ltd |
Issue Date: | 2004 |
ISSN: | 0951-4198 1097-0231 |
Statement of Responsibility: | Vita M. Maselli, Craig S. Brinkworth, John H. Bowie, Michael J. Tyler |
Abstract: | The amino acid sequences of seven signiferin peptides are provided by consideration of tandem mass spectrometric (MS/MS) data for the respective MH+ and [M--H]- precursor ions. These methods do not differentiate between isomeric residues Leu and Ile; these were identified using the Edman degradation technique. The sequence of signiferin 1, a new smooth muscle contracting neuropeptide (RLCIPYIIPC-OH) containing a disulfide bridge, is best determined using the collision-induced dissociation (CID) spectrum of the [M--H]- anion. The initial fragmentation of this system involves loss of H2S2, which furnishes an open-chain system that is readily sequenced using the alpha and beta backbone cleavage anions. |
Keywords: | Skin Animals Ranidae Ions Peptides Neuropeptides Amphibian Proteins Spectrometry, Mass, Electrospray Ionization Sequence Analysis, Protein Immunity Amino Acid Sequence Molecular Sequence Data |
Description: | The definitive version may be found at www.wiley.com |
Provenance: | Published Online: 13 Aug 2004 |
DOI: | 10.1002/rcm.1602 |
Published version: | http://www3.interscience.wiley.com/journal/109594071/abstract |
Appears in Collections: | Aurora harvest 2 Chemistry publications |
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