Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/133478
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Type: | Journal article |
Title: | A versatile strategy for isolating a highly enriched population of intestinal stem cells |
Author: | Nefzger, C.M. Jardé, T. Rossello, F.J. Horvay, K. Knaupp, A.S. Powell, D.R. Chen, J. Abud, H.E. Polo, J.M. |
Citation: | Stem Cell Reports, 2016; 6(3):321-329 |
Publisher: | Cell Press |
Issue Date: | 2016 |
ISSN: | 2213-6711 2213-6711 |
Statement of Responsibility: | Christian M. Nefzger, Thierry Jardé, Fernando J.Rossello, Katja Horvay, Anja S.Knaupp, David R.Powell ... et al. |
Abstract: | The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. Used on reporter-free mice, this strategy allows the isolation of functional, transcriptionally distinct ISCs uncompromised by Lgr5 haploinsufficiency. |
Keywords: | Intestinal Mucosa Cells, Cultured Animals Mice, Inbred C57BL Mice Receptors, G-Protein-Coupled Male Adult Stem Cells Primary Cell Culture |
Rights: | © 2016 The Authors. Published by Elsevier Inc. |
DOI: | 10.1016/j.stemcr.2016.01.014 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1061883 |
Published version: | http://dx.doi.org/10.1016/j.stemcr.2016.01.014 |
Appears in Collections: | Medical Sciences publications |
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hdl_133478.pdf | Published version | 2.26 MB | Adobe PDF | View/Open |
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