Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/132955
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dc.contributor.author | Floerchinger, A. | - |
dc.contributor.author | Murphy, K.J. | - |
dc.contributor.author | Latham, S.L. | - |
dc.contributor.author | Warren, S.C. | - |
dc.contributor.author | McCulloch, A.T. | - |
dc.contributor.author | Lee, Y.-K. | - |
dc.contributor.author | Stoehr, J. | - |
dc.contributor.author | Mélénec, P. | - |
dc.contributor.author | Guaman, C.S. | - |
dc.contributor.author | Metcalf, X.L. | - |
dc.contributor.author | Lee, V. | - |
dc.contributor.author | Zaratzian, A. | - |
dc.contributor.author | Da Silva, A. | - |
dc.contributor.author | Tayao, M. | - |
dc.contributor.author | Rolo, S. | - |
dc.contributor.author | Phimmachanh, M. | - |
dc.contributor.author | Sultani, G. | - |
dc.contributor.author | McDonald, L. | - |
dc.contributor.author | Mason, S.M. | - |
dc.contributor.author | Ferrari, N. | - |
dc.contributor.author | et al. | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Cell Reports, 2021; 36(11):109689-1-109689-e6 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | https://hdl.handle.net/2440/132955 | - |
dc.description.abstract | Assessing drug response within live native tissue provides increased fidelity with regards to optimizing efficacy while minimizing off-target effects. Here, using longitudinal intravital imaging of a Rac1-Förster resonance energy transfer (FRET) biosensor mouse coupled with in vivo photoswitching to track intratumoral movement, we help guide treatment scheduling in a live breast cancer setting to impair metastatic progression. We uncover altered Rac1 activity at the center versus invasive border of tumors and demonstrate enhanced Rac1 activity of cells in close proximity to live tumor vasculature using optical window imaging. We further reveal that Rac1 inhibition can enhance tumor cell vulnerability to fluid-flow-induced shear stress and therefore improves overall anti-metastatic response to therapy during transit to secondary sites such as the lung. Collectively, this study demonstrates the utility of single-cell intravital imaging in vivo to demonstrate that Rac1 inhibition can reduce tumor progression and metastases in an autochthonous setting to improve overall survival. | - |
dc.description.statementofresponsibility | Alessia Floerchinger … Michael S. Sasmuel … et al. | - |
dc.language.iso | en | - |
dc.publisher | Elsevier BV | - |
dc.rights | © 2021 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | - |
dc.source.uri | http://dx.doi.org/10.1016/j.celrep.2021.109689 | - |
dc.subject | Cell Line, Tumor | - |
dc.subject | Animals | - |
dc.subject | Mice, Inbred BALB C | - |
dc.subject | Humans | - |
dc.subject | Mice | - |
dc.subject | Breast Neoplasms | - |
dc.subject | Lung Neoplasms | - |
dc.subject | Pyrimidines | - |
dc.subject | Aminoquinolines | - |
dc.subject | rac1 GTP-Binding Protein | - |
dc.subject | Fluorescence Resonance Energy Transfer | - |
dc.subject | Biosensing Techniques | - |
dc.subject | Signal Transduction | - |
dc.subject | Cell Movement | - |
dc.subject | Cell Survival | - |
dc.subject | Shear Strength | - |
dc.subject | Female | - |
dc.title | Optimizing metastatic-cascade-dependent Rac1 targeting in breast cancer: guidance using optical window intravital FRET imaging | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.celrep.2021.109689 | - |
dc.relation.grant | NHMRC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Samuel, M.S. [0000-0001-7880-6379] | - |
Appears in Collections: | Medicine publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_132955.pdf | Published Version | 4.58 MB | Adobe PDF | View/Open |
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