Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/126252
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Type: Journal article
Title: In vitro analysis of the effect of Flightless I on murine tenocyte cellular functions
Author: Jackson, J.E.
Kopecki, Z.
Anderson, P.J.
Cowin, A.J.
Citation: Journal of orthopaedic surgery and research, 2020; 15(1):170
Publisher: BMC
Issue Date: 2020
ISSN: 1749-799X
1749-799X
Statement of
Responsibility: 
Jessica E. Jackson, Zlatko Kopecki, Peter J. Anderson and Allison J. Cowin
Abstract: BACKGROUND:Healing of tendons after injury involves the proliferation of tenocytes and the production of extracellular matrix; however, their capacity to heal is limited by poor cell density and limited growth factor activity. Flightless I (Flii) has previously been identified as an important regulator of cellular proliferation and migration, and the purpose of this study was to evaluate the effect of differential Flii gene expression on tenocyte function in vitro. METHODS:The role of Flii on tenocyte proliferation, migration, and contraction was assessed using established assays. Tenocytes from Flii+/-, wild-type, and Flii overexpressing mice were obtained and the effect of differential Flii expression on migration, proliferation, contraction, and collagen synthesis determined in vitro. Statistical differences were determined using unpaired Student's t test and statistical outliers were identified using the Grubbs' test. RESULTS:Flii overexpressing tenocytes showed significantly improved migration and proliferation as well as increased collagen I secretion. Explanted tendons from Flii overexpressing mice also showed significantly elevated tenocyte outgrowth compared to Flii+/- mice. In contrast to its role in dermal wound repair, Flii positively affects cellular processes in tendons. CONCLUSIONS:These findings suggest that Flii could be a novel target for modulating tenocyte activity and improving tendon repair. This could have significant clinical implications as novel therapeutic targets for improved healing of tendon injuries are urgently needed.
Keywords: Flightless I; Flii; Healing; Tendon
Rights: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
RMID: 1000021132
DOI: 10.1186/s13018-020-01692-9
Grant ID: http://purl.org/au-research/grants/nhmrc/1102617
Appears in Collections:Orthopaedics and Trauma publications

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