Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/125199
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Fabrication of a cartilage patch by fusing hydrogel-derived cell aggregates onto electrospun film |
Author: | Zhang, J. Yun, S. Du, Y. Zannettino, A.C.W. Zhang, H. |
Citation: | Tissue Engineering Part A, 2020; 26(15-16):1-9 |
Publisher: | Mary Ann Liebert |
Issue Date: | 2020 |
ISSN: | 1937-3341 1937-335X |
Statement of Responsibility: | Jiabin Zhang, Seonho Yun, Yuguang Du, Andrew C.W. Zannettino and Hu Zhang |
Abstract: | Irregular defects at sites of degenerative cartilage often accompany osteoarthritis (OA). The development of novel cells/biomaterials-based cartilage tissue engineering methods to address these defects may provide a durable approach to hinder the development of OA. In the present study, we fabricated a neo-cartilage patch by fusing cell aggregates onto a biodegradable nanofiber film for degenerative cartilage repair. Human mesenchymal stem/stromal cell (MSC) aggregates were prepared and induced for chondrogenesis in a thermosensitive hydrogel, poly (<i>N</i>-isopropylacrylamide-co-acrylic acid) (p(NIPAAm-AA)). Cell migration mediated the formation of cell aggregates in the thermosensitive hydrogel and led to a cell-dense hollow shell structure. The chondrocytes derived from MSC aggregates in the hydrogel were evidenced by the expression of chondrogenesis-related genes and extracellular matrices (ECMs). They were fused onto an electrospun film by mechanical force and spatial confinement to generate a neo-cartilage patch. The fabricated neo-cartilage patches may be able to integrate into the irregular defects under compressive stresses and achieve cartilage regeneration <i>in vivo</i>. |
Keywords: | Hydrogel; chondrogenesis; human mesenchymal stem/stromal cell; electrospinning; cell aggregates; cartilage regeneration |
Description: | Published Online:19 Mar 2020 |
Rights: | ©t 2020, Mary Ann Liebert, Inc., publishers |
DOI: | 10.1089/ten.TEA.2019.0318 |
Grant ID: | http://purl.org/au-research/grants/arc/DP160104632 |
Published version: | http://dx.doi.org/10.1089/ten.tea.2019.0318 |
Appears in Collections: | Aurora harvest 8 Chemical Engineering publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.